Antibiotics, Buffers and Supplements

Gibco™ Penicillin-Streptomycin (10,000 U/mL)

For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria

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Gibco™ Trypsin-EDTA (0.05%), Phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

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Gibco™ Penicillin-Streptomycin-Glutamine (100X)

for prevention from bacterial contamination in cell cultures due to their effective combined action against gram-positive and gram-negative bacteria.

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Gibco™ GlutaMAX™ Supplement

An alternative to L-glutamine, with increased stability that improves cell health

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Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

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Gibco™ TrypLE Express Enzyme (1X), No Phenol Red

Unlike porcine trypsin, free of animal-derived components

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Gibco™ Hygromycin B (50mg/mL)

Water-soluble antibiotic purified from bacterium Streptomyces hydroscopicus

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Lonza™ BioWhittaker™ Antibiotics: Penicillin-Streptomycin Mixtures

Used as a broad spectrum bacteriostatic and bactericidal, with activity against gram negative and gram-positive organisms including yeast and mold. BioWhittaker™ Antibiotics: Penicillin-Streptomycin Mixtures are manufactured in accordance with cGMP regulations.

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Lonza™ BioWhittaker™ Hank's Balanced Salt Solutions (1X)

Used in wide variety of tissue culture applications. BioWhittaker™ Hank's Balanced Salt Solutions (1X) is used to maintain a physiological pH for cells maintained in non-CO2 conditions, diluting cells before counting, washing cells before dissociating and transporting cells or tissues.

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Gibco™ HEPES (1M)

A zwitterionic organic chemical buffering agent commonly used in cell culture media

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Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

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Gibco™ Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

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Corning™ Matrigel™ Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, LDEV-Free

Solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. Corning™ Matrigel™ Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, LDEV-Free is tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

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Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

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Lonza™ BioWhittaker™ Phosphate Buffered Solution (1X)

Serves as a wash solution in a wide variety of laboratory applications.  BioWhittaker™ Phosphate Buffered Solution (1X) Phosphate Buffered Saline is manufactured in accordance with cGMP regulations.

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Gibco™ Trypsin-EDTA (0.25%), Phenol red

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

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Gibco™ Blasticidin S HCl (10mg/mL), Sterile

Selective antibiotic in concentration range of 50G–100μg/mL

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Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses

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Lonza™ BioWhittaker™ Dulbecco's Phosphate Buffered Saline (1X) w/o Calcium and Magnesium

Used to provide buffer system to maintain cell culture media in the physiological pH range of 7 to 7.6.

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Ampicillin Sodium Salt (Crystalline Powder), Fisher BioReagents

CAS: 69-52-3 Molecular Formula: C16H21N3NaO4S Molecular Weight (g/mol): 374.411 InChI Key: BSFVNXCYXDYHOD-ZQDFAFASSA-N Synonym: Ampicillin PubChem CID: 131673879 IUPAC Name: (2S,5R,6R)-6-[[(2R)-2-amino-2-phenylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid;molecular hydrogen;sodium SMILES: [HH].CC1(C(N2C(S1)C(C2=O)NC(=O)C(C3=CC=CC=C3)N)C(=O)O)C.[Na]

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Gibco™ EMEM

Eagle's Minimum Essential Medium (EMEM)

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Carbenicillin (Disodium Salt), Fisher BioReagents

CAS: 4800-94-6 Molecular Formula: C17H16N2Na2O6S Molecular Weight (g/mol): 422.363 InChI Key: RTYJTGSCYUUYAL-YCAHSCEMSA-L Synonym: α-Carboxybenzylpenicillin PubChem CID: 20933 ChEBI: CHEBI:34609 IUPAC Name: disodium;(2S,5R,6R)-6-[(2-carboxylato-2-phenylacetyl)amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylate SMILES: CC1(C(N2C(S1)C(C2=O)NC(=O)C(C3=CC=CC=C3)C(=O)[O-])C(=O)[O-])C.[Na+].[Na+]

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Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

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Lonza™ BioWhittaker™ Dulbeccos Phosphate Buffered Saline (10X)

Used as a buffer system for cell culture media.  BioWhittaker™ Dulbeccos Phosphate Buffered Saline (10X) is manufactured in accordance with cGMP regulations and cell culture-tested to assure compatibility.

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Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate), Powder

Produced by bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells

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