Biological Buffers
Thermo Scientific™ DNA Gel Loading Dye (6X)
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.
Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled.
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Thermo Scientific™ Blocker™ BLOTTO in TBS
Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.
Gibco™ HEPES (1M)
A zwitterionic organic chemical buffering agent commonly used in cell culture media
Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled
HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™
Commonly used buffering agent
Invitrogen™ NuPAGE™ Antioxidant
Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Invitrogen™ Nuclease-Free Water (not DEPC-Treated)
Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving.
Invitrogen™ PureLink™ RNase A (20 mg/mL)
PureLink™ RNase A (ribonuclease A) is a bovine pancreatic ribonuclease that cleaves single-stranded RNA.
Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)
Ready-to-use reagent to reduce protein samples for protein gel electrophoresis
Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)
Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.
Invitrogen™ NuPAGE™ Transfer Buffer (20X)
Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting
Invitrogen™ 20X Bolt™ MES SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
PIPES, 98%, Thermo Scientific Chemicals
CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N Synonym: 2,2'-piperazine-1,4-diyl bis ethanesulphonic acid, 1,4-piperazine-diethanesulfonic acid, piperazine-1,4-bis 2-ethanesulfonic acid, 2-4-2-sulfoethyl piperazin-1-yl ethanesulfonic acid, unii-g502h79v6l, 1,4-piperazinebis ethanesulfonic acid, 2,2'-piperazine-1,4-diyl diethanesulfonic acid, piperazine-n,n'-bis 2-ethanesulfonic acid, 1,4-piperazinediethanesulfonic acid, pipes PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.
Invitrogen™ DEPC-Treated Water
Ambion DEPC-treated water is certified nuclease-free. DEPC-treated water is autoclaved pre- and post-packaging to ensure sterility and inactivation of DEPC.
Invitrogen™ GlycoBlue™ Coprecipitant (15 mg/mL)
GlycoBlue Coprecipitant consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate, which is useful as a nucleic acid coprecipitant.
Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)
Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Invitrogen™ Sodium Acetate (3 M), pH 5.5, RNase-free
Invitrogen Molecular biology grade, 3M Sodium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ NativePAGE™ Running Buffer (20X)
Used to make the NativePAGE Cathode and Anode Running Buffers
BD Antibody Diluent
For acetone-fixed, frozen tissue sections and formalin-fixed, paraffin-embedded tissue sections
Invitrogen™ TBE (10X), RNase-free
Molecular biology grade, Invitrogen 10X TBE solution is supplied in four bottles containing 1 L each. The buffer is certified RNase-free, economical, and ready-to-use.
Invitrogen™ Proteinase K, recombinant
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)
Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100 to 200 μg/mL.
Thermo Scientific™ Pierce™ LDS Sample Buffer, Non-Reducing (4X)
Nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.
Invitrogen™ 20X Bolt™ MOPS SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Thermo Scientific™ Pierce™ Gentle Ag/Ab Elution Buffer, pH 6.6
Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions.