Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Product Code	Brand	Quantity
10485315	Thermo Scientific™ 21062	30 mL
10057103	Thermo Scientific™ 21059	500 mL
11874524	Thermo Scientific™ 21063	5 L

Description

Thermo Scientific™ Restore Western Blot Stripping Buffer safely and effectively removes primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Performing gel electrophoresis and duplicate immunoblot assays to test new primary antibodies or antibody concentrations is time-consuming and expensive. Restore Western Blot Stripping Buffer eliminates this waste when detecting immunoblots with chemiluminescent Western blotting substrates. The reagent allows clean and efficient removal of primary and secondary antibodies from immunoblots without removing or damaging the immobilized antigen. This allows blots to be stripped and reprobed with confidence.

Highlights:

• Saves time – no need to re-run gels and blots

• Saves costly sample – re-probe the membrane using the same target sample

• Effective – formulation is more efficient at stripping antibodies than homemade buffers

• Gentle – does not damage the target antigen during stripping allowing efficient reprobing

• Odor-free – no mercaptans means no acrid odors

• Economical – less expensive than other commercial stripping buffers

Reuse a nitrocellulose or PVDF blot to detect a different target with a different primary antibody; Reprobe a blot to correct or optimize antibody concentrations that were ineffective the first time.

Specifications

• Form: Liquid
• Product Line: Restore™
• Content And Storage: Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature.

Safety and Handling

Product Identifier

• Restore Western Blot Stripping Buffer

Signal Word

• Danger

Hazard Category

• Substances and mixtures corrosive to metals Category 1
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 1

Hazard Statement

• H290-May be corrosive to metals.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P234-Keep only in original container.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P406-Store in corrosive resistant/ container with a resistant inner liner.

Supplemental information

• MIXTURE LIST-Contains : Organo Phosphine

For Research Use Only. Not for use in diagnostic procedures.