Biological Buffers
Thermo Scientific™ 6X DNA Loading Dye
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene
Invitrogen™ Nuclease-Free Water (not DEPC-Treated)
NUCLEASE-FREE WATER 5 X 100 MLAmbion® Nuclease-free water has not been treated
Thermo Scientific™ Blocker™ BLOTTO Blocking Buffer
Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods. BLOCKER BLOTTO IN TBS, 1 L, MA: 375304°C
Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)
LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C
Thermo Scientific™ Blocker™ BSA Blocking Buffers
Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods. BLOCKER BSA IN TBS(10X)125ML
Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF
Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF
Invitrogen™ Novex™ NuPAGE™ Antioxidant
Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT
Invitrogen™ UltraPure™ RNase A (20mg/mL)
Isolates RNA-free DNA 10 ML RNASE A 10ML SHELF LIFE = 3650 PACKINGinstructions = RRR Storage = Fridge 4C
HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents
Commonly used buffering agent 1LT HEPES Buffer, 1M Solution, pH 7.3, for Molecular Biology
Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)
Ready-to-use reagent to reduce protein samples for protein gel electrophoresis SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(R)10mL Store at 2-8C
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 30 ML WESTERN STRIPPING REAGENT, RESTORE 30ML
Thermo Scientific™ Pierce™ LDS 4X Nonreducing Sample Buffer
Nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes. LDS SAMPLE BUFFER NON-REDUCING 4X
Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4
Dilute to desired concentration 10X PBS BUFFER, PH 7.4 500 MLMolecular biology grade, Ambion® PBS 10X, pH 7.4
Invitrogen™ DEPC-Treated Water
4000ML DEPC-TREATED WATER 4 X 1000 ML
Invitrogen™ Proteinase K Solution (20mg/mL)
General digestion of proteins PROTEINASE K (20MG/ML)5X1.25ML5 tubes
Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL
Invitrogen™ EDTA (0.5M), pH 8.0
Ready-to-use, certified RNase-free 1 LT 0.5 M EDTA, PH 8.0 1L STORE AT ROOM TEMPERATUre
Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)
Blue dye increases pellet visibility GLYCO BLUE COPRECIP 5 X 300ULAmbion® GlycoBlue consists of a blue dye
Invitrogen™ Proteinase K
Remains active under various conditions PROTEINASE K 100MG
Invitrogen™ NaCl (5M)
Ready-to-use, certified RNase-free 5M NACL 500 MLAmbion® Molecular biology grade, 5 M NaCl
Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)
Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL
Applied Biosystems™ Platinum Multiplex PCR Master Mix
Template amplification from 50bp to 2.5kb 2X PLATINUM MULTIPLEX MMX
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 5KG HEPES (Fine White Crystals) for Molecular Biology,
Invitrogen™ AccuPrime™ Pfx SuperMix
High-fidelity, high-specificity amplification of DNA fragments 200 RXN PFX SUPERMIX 200 REACTIONS STORE AT -20C
Invitrogen™ RT-PCR Grade Water, Not DEPC-treated
RT-PCR GRADE WATER 10 X 1.7MLAmbion® RT-PCR grade water is tested for
Thermo Scientific™ FastDigest Buffer (10X)
FastDigest Buffer (10X) X10 BUFF FASTDIGEST 1ML
Thermo Scientific™ Pierce™ Binding and Elution Buffers for IgG Purification
Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions. PROTEIN G IGG BINDING BUFFER 1L
Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)
Premixed, safer, and easier alternative to preparing crystalline phenol solutions 400 ML ACID-PHENOL:CHLOROFORM, PH 4.5 (WITH IAA,125:24:1) 400mL Store at 4°C
Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)
Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting 1 LT Transfer buffer NuPAGE(r) (20X), liquid 1L