Enzymes and Inhibitors

Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA)

Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA). TBE BUFFER 1L 10X

Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA). 1 LT 50X TAE BUFFER (TRIS-ACETATE-EDTA), 1X CONCENtration includes 40 mM Tris, 20 mM acetic acid, 1

Thermo Scientific™ EcoRI

The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer. ECORI 50U/UL 25000U

Thermo Scientific™ FastDigest SphI (PaeI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest PaeI, 20µL

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. 250 UL BOVINE SERUM ALBUMIN (BSA), 20MG/ML, 5MG(0.25mL), supplied in 10 mM Tris-HCl (pH7.4 at

Thermo Scientific™ FastDigest™ EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST ECORI 500UL

Thermo Scientific™ Esp3I (BsmBI)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI). ESP3I (BSMBI) 10U/UL 1000U

Thermo Scientific™ SspI

The SspI restriction enzyme recognizes AAT^ATT sites and cuts best at 37°C in G buffer. 2500 UNIT RESTRICTION ENZYME, CONVENTIONAL, SSPI,10u/uL, cuts AAT¬ATT at 37°C (suppliedwith 1mL of

Thermo Scientific™ DpnI

Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI). DPNI 10U/UL 1000U

Thermo Scientific™ FastDigest™ XhoI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. X3 FASTDIGEST XHOI 400UL

Thermo Scientific™ FastDigest™ SpeI (BcuI)

FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI). Restriction enzyme, FastDigest BcuI, 50µL

Thermo Scientific™ FastDigest™ NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI). 250 UL RESTRICTION ENZYME, FASTDIGEST, NOTI, CUTSGC¬GGCCGC, 1µL cleaves 1µg DNA in 5 minutes at

Thermo Scientific™ PscI (PciI)

The PscI (PciI) restriction enzyme recognizes A^CATGT sites and cuts best at 37°C in Tango buffer (Isoschizomers: PciI). RESTRICTION ENZYME, CONVENTIONAL, PSCI (PCII),10u/ul, cuts A¬CATGT at 37°C (supplied with 1mL

Thermo Scientific™ BamHI

The BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer. BAMHI 50U/UL 20000U

Thermo Scientific™ XhoI

Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI). XHOI 10U/UL 5X2000U

Thermo Scientific™ TaqI

The TaqI restriction enzyme recognizes T^CGA sites and cuts best at 65°C in its own unique buffer. 3000 UNIT RESTRICTION ENZYME, CONVENTIONAL, TAQI,10u/uL, cuts T¬CGA at 65°C (supplied with 1mL of

Thermo Scientific™ FastDigest SalI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST SALI 200UL

Thermo Scientific™ FastDigest™ XbaI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 750 UL RESTRICTION ENZYME, FASTDIGEST, XBAI, CUTST¬CTAGA, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ HindIII

The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer. 25000 UNIT RESTRICTION ENZYME, CONVENTIONAL,HindIII, 50u/uL,HC, cuts A¬AGCTT at 37°C (supplied

Thermo Scientific™ BsuRI (HaeIII)

Cut at GG^CC sites with BsuRI (HaeIII) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: BshFI, BsnI, BspANI, HaeIII, PhoI). RESTRICTION ENZYME, CONVENTIONAL, BSURI (HAEIII),10u/µL cuts GG¬CC at 37°C (supplied with 1mL of

Thermo Scientific™ XbaI

Cut at T^CTAGA sites with XbaI restriction enzyme, which functions best at 37°C in Tango buffer. XBAI 10U/UL 5X1500U

Thermo Scientific™ KpnI

The KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Asp718I). RESTRICTION ENZYME, CONVENTIONAL, KPNI, 10U/UL,cuts GGTAC¬C at 37°C (suppliedwith 1mL of 10X

Thermo Scientific™ FastDigest SexAI (CsiI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest CsiI, 50µL

Thermo Scientific™ MspI (HpaII)

The MspI (HpaII) restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer. MSPI (HPAII) 10U/UL 5X3000U

Thermo Scientific™ 10X T4 DNA Ligase Buffer

Safely ligate samples with this buffer, for use with T4 DNA Ligase and tested for the absence of endo- and exodeoxyribonucleases and ribonucleases. X10 T4 DNA LIGASE 1.5ML

Thermo Scientific™ FastDigest BbsI (BpiI)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I). Restriction enzyme, FastDigest BpiI, 20µL

Thermo Scientific™ FastDigest™ HindIII

The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST HINDIII 500UL

Thermo Scientific™ FastDigest KpnI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST KPNI 300UL

Thermo Scientific™ Hin6I (HinP1I)

Cut at G^CGC sites with Hin6I (HinP1I) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AspLEI, BstHHI, CfoI, HinP1I, HspAI). 2000 UNIT RESTRICTION ENZYME, CONVENTIONAL, HIN6I(HinP1I), 10u/uL, cuts G¬CGC at 37°C (supplied

Thermo Scientific™ FastDigest AgeI (BshTI)

Cut at A^CCGGT sites with Thermo Scientific™ FastDigest™ AgeI (BshTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest BshTI, 20µL

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