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Thermo Scientific™ Pierce™ Binding and Elution Buffers for IgG Purification

Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions.

£44.97 - £291.00

Specifications

Target IgG
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Products 13
Product Code Brand Description Quantity Price Quantity & Availability  
Product Code Brand Description Quantity Price Quantity & Availability  
10057003 View Documents Thermo Scientific™
21012
Pierce Gentle Ag/Ab Binding Buffer, pH 8.0 3.75L £220.00
3.75 L View alternative products
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Estimated Shipment date
from Supplier 17-12-2019
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10209973 View Documents Thermo Scientific™
21011
Pierce Protein G IgG Binding Buffer 3.75L £231.00
3.75 L View alternative products
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Estimated Shipment date
from Supplier 19-12-2019
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10290544 View Documents Thermo Scientific™
21027
Pierce Gentle Ag/Ab Elution Buffer, pH 6.6 500mL £106.00
500mL View alternative products
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EU Stock 7 Please to purchase this item. Need a web account? Register with us today!
10017053 View Documents Thermo Scientific™
21013
Pierce Gentle Ag/Ab Elution Buffer, pH 6.6 3.75L £291.00
3.75 L View alternative products
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EU Stock 7 Please to purchase this item. Need a web account? Register with us today!
10188163 View Documents Thermo Scientific™
21030
Pierce Gentle Ag/Ab Binding and Elution Buffer Kit 200mL kit £111.00
100mL View alternative products
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EU Stock 2 Please to purchase this item. Need a web account? Register with us today!
11806804 View Documents Thermo Scientific™
21020
Pierce Gentle Ag/Ab Binding Buffer, pH 8.0 1L £118.00
1000mL View alternative products
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EU Stock 3 Please to purchase this item. Need a web account? Register with us today!
13454229 View Documents Thermo Scientific™
21028
Pierce IgG Elution Buffer, pH 2.0 1L £80.50
1 L View alternative products
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EU Stock 5 Please to purchase this item. Need a web account? Register with us today!
11881044 View Documents Thermo Scientific™
21009
Pierce IgG Elution Buffer 3.75L £192.00
3.75 L View alternative products
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EU Stock 8 Please to purchase this item. Need a web account? Register with us today!
11849410 View Documents Thermo Scientific™
54200
Pierce Protein A/G IgG Binding Buffer 240mL £44.97
240mL View alternative products
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EU Stock 2 Please to purchase this item. Need a web account? Register with us today!
10763495 View Documents Thermo Scientific™
21001
Pierce Protein A IgG Binding Buffer 1L £83.00
1 L View alternative products
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EU Stock 4 Please to purchase this item. Need a web account? Register with us today!
10455944 View Documents Thermo Scientific™
21019
Pierce Protein G IgG Binding Buffer 1L £61.75
1 L View alternative products
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EU Stock 6 Please to purchase this item. Need a web account? Register with us today!
10433805 View Documents Thermo Scientific™
21004
Pierce IgG Elution Buffer 1L £76.75
1 L View alternative products
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EU Stock 3 Please to purchase this item. Need a web account? Register with us today!
10751965 View Documents Thermo Scientific™
21007
Pierce Protein A IgG Binding Buffer 3.75L £253.00
3.75 L View alternative products
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EU Stock 4 Please to purchase this item. Need a web account? Register with us today!
Description

Description

These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.

The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.

Finally, the Mouse IgG1 Mild Elution Buffer, when used in combination with Protein A resin, enables selective elution and separation of the IgG1 subclass from total bound mouse IgG.

Highlights:

  • Three optimized binding buffers – for Protein A, Protein G or Protein A/G

  • Two elution buffer options – acidic (pH 2.8) or high salt (near-neutral)

  • Special system for mouse IgG1 – selective, two-stage elution from Protein A agarose

Item-Specific Features:

  • Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA

  • Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA

  • Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide

  • IgG Elution Buffer: pH 2.8; amine-based

  • Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide

  • Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)

  • Mouse IgG1 Mild Elution Buffer: pH 6.0; contains 0.02% sodium azide

Includes:

Kits contain both binding and elution buffers

Specifications

Specifications

IgG