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IBA Solutions for Life Science™ Anidrotetraciclina

CAS: 1665-56-1 Formula molecolare: C22H22N2O7 Molecular Weight (g/mol): 426.43 InChI Key: JGRMIMMCZBOWCW-LCGGBVFXSA-N Sinonimo: anhydrotetracycline, 5a,6-anhydrotetracycline, 4s-anhydrotetracycline, cxcveerymjzmmm-docrcchosa-n, 4s,4as,12as-4-dimethylamino-3,10,11,12a-tetrahydroxy-6-methyl-1,12-dioxo-1,4,4a,5,12,12a-hexahydrotetracene-2-carboxamide, anhydrotetracycline hydrochloride, 2-naphthacenecarboxamide, 1,4,4a,5,12,12a-hexahydro-4-dimethylamino-1,12-dioxo-6-methyl-3,10,11,12a-tetrahydroxy-, 4s-4-alpha,4a-alpha,12a-alpha, 2-naphthacenecarboxamide,4-dimethylamino-1,4,4a,5,12,12a-hexahydro-3,10,11,12a-tetrahydroxy-6-methyl-1,12-dioxo-, 4s,4as,12as, anhydrotetracyclin, tdc PubChem CID: 54675758 50 MG ANHYDROTETRACYCLINE 50MG STORE IN -20CFreezer

IBA Lifesciences™ Coniugato Strep-Tactin-HRP

Used for fast and direct detection of Strep-tag™II fusion proteins transferred to a blotting membrane or coated to a microplate. IBA Lifesciences™ Strep-Tactin-HRP Conjugate is suitable for chromogenic and chemiluminescence reactions. 500 UL STREP-TACTIN(R) HRP CONJUGATE 0.5ML STOREin 4C Fridge

IBA Solutions for Life Science™ Tampone di rigenerazione Strep-tag™, 10X

100 ML STREP-TAG(R) REGENERATION BUFFER WITH HABA(10x Buffer R) 100mL Store in 4C Fridge

IBA GmbH™ Strep-Tactin™ APC for MHC I Streptamers™

IBA GmbH™ Strep-Tactin™ APC for MHC I Streptamers™ is for staining/FACS isolation of antigen-specific cytotoxic T cells (CD8). Strep-Tactin APC for MHC I Streptamers; 50 µl

IBA Lifesciences™ Magnetic Separator

Aids fast purification of Strep-tag™ proteins. Magnetic Separator for 24 rxntubes, 1piece

IBA GmbH™ Nylon Filter Tubes for Streptamer™ Cell Isolation

IBA GmbH™ Nylon Filter Tubes for Streptamer™ Cell Isolation can be used for a variety of applications like removal of cell clumps and debris. Nylon filter tubes for cell isolation; 10 pieces

IBA Solutions For Life Science™ 2'-Amino-2'-dUTP IBA

500 NMOL 2'-Amino-2'-dUTP 0.5µmol Store in Freezer

IBA Solutions For Life Science 5'-Cyanine 5-ApG Transcription Starter

A DNA/RNA dinucleotide (oligonucleotide for 5´-labeled transcripts) used by RNA polymerases as an initiator for RNA synthesis and therefore very useful for transcription analysis. 5'-Cyanine 5-ApG

IBA Solutions For Life Science™ ApA-dinucleotide IBA

5 MG ApA-dinucleotide 5mg Store in Freezer at -20C

IBA Solutions For Life Science™ UpU-dinucleotide IBA

1 MMOL UpU-dinucleotide 1µmol Store in Freezer at

IBA Solutions For Life Science StarGate™ pESG-IBA Acceptor Vectors for Mammalian Expression

Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen. pESG-IBA123; 5µg

IBA GmbH™ Mammalian Expression Vector with No Secretion

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA GmbH™ Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR). pCSG-IBA65; 5µg

IBA Solutions For Life Science StarGate™ pLSG-IBA Acceptor Vectors for Baculo

Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination. pLSG-IBA33; 5µg

IBA Solutions For Life Science™ Strep-Tactin™ Superflow™, 50% Suspension

Used for purification of recombinant Strep-tag™ proteins under gravity flow and low pressure or FPLC 50 ML Strep-Tactin(R) Superflow(R), 50 % suspensio

IBA Solutions For Life Science™ ApA-dinucleotide IBA

10 MG ApA-dinucleotide 10mg Store in Freezer at

IBA GmbH™ Strep-Tactin™XT Superflow™ 50% Suspension

IBA GmbH™ Strep-Tactin™XT Superflow™ 50% Suspension can be used to pack your own columns for protein purification. 50ml Strep-Tactin XT Superflow 50% suspension

IBA Solutions For Life Science StarGate™ pPSG-IBA Acceptor Vectors for E. coli Expression

Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins. pPSG-IBA43; 5µg

IBA Solutions For Life Science™ CpG-dinucleotide IBA

1 MG CpG-dinucleotide 1mg Store in Freezer at -20C

IBA Solutions For Life Science pdCpA Dinucleotid Transcription Starter

An RNA dinucleotide (oligonucleotide for 5´-labeled transcripts) used by RNA polymerases as an initiator for RNA synthesis and therefore very useful for transcription analysis. pdCpA Dinucleotid

IBA GmbH CD4 Isolation Kit for FABian&trade, Mouse

For automated positive cell separation. The Isolation Kit for FABian&trade provides label-free target cells without the use of high affinity antibodies and magnetic beads CD4 Isolation Kit for FABian®, mouse, 1 Kit

IBA Solutions For Life Science™ GpA-dinucleotide IBA

5 MG GpA-dinucleotide 5mg Store in Freezer at -20C

IBA Solutions For Life Science™ CpC-dinucleotide IBA

10 MG CpC-dinucleotide 10mg Store in Freezer at

IBA Solutions For Life Science™ GpG-dinucleotide IBA

10 MG GpG-dinucleotide 10mg Store in Freezer at

IBA Solutions For Life Science™ 2-Thio-CTP IBA

500 NMOL 2-Thio-CTP 0.5µmol Store in Freezer at

IBA Solutions For Life Science Cyanine 5-ApG-PH Transcription Starter

A DNA/RNA dinucleotide (oligonucleotide for 5´-labeled transcripts) used by RNA polymerases as an initiator for RNA synthesis and therefore very useful for transcription analysis. Cyanine 5-ApG-PH

IBA Solutions For Life Science™ Double Stranded Negative Control RNA (Mock)

5 NMOL Double stranded negative control RNA (mock)

IBA Solutions for Life Science™ MagStrep “type 3” XT Beads 5% Suspension

Have high binding capacity combined with very low non-specific protein binding 2ML MagStrep ''type3'' XT beads , 5% suspension

IBA Solutions For Life Science 2'-Amino-2'-dCTP

A modified nucleoside-triphosphate (3´-modified oligos) that prevents nuclease degradation of RNAs. 2'-Amino-2'-dCTP

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Featured Products

Twin-Strep-tag™/Strep-Tactin™XT


Twin-Strep-tag

The purification system with the highest affinity – one tag for expression, purification, detection and immobilization

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Immobilization with near covalent binding affinity


Immobilization with near covalent binding affinity

Twin-Strep-tag™ Capture Kit for SPR experiments »

  • High affinity in low pM range enables long term measurements (T1/2 = 13 h)
  • Chip can be easily regenerated

High affinity meets high capacity!


High affinity meets high capacity!

Strep-Tactin™XT Superflow™ high capacity »

  • Higher protein yields compared to all other Strep-Tactin™ resins
  • Dynamic binding capacity of ~15 mg protein per ml resin
  • Elution of concentrated protein due to small elution volumes
  • Cost efficent: small price for more protein

Try our new high affinity Strep-tag™ system


Try our new high affinity Strep-tag® system

Starter Kit consists of:

  • Strep-Tactin™XT Superflow™ gravity flow column
  • Buffer W (Strep-Tactin™/Strep-Tactin™XT Wash Buffer)
  • Buffer BXT (Strep-Tactin™XT Elution Buffer with Biotin)
  • Strep-Tactin™ conjugated with HRP (for Western blot detection)
  • Short protocol
Cell
IBS's Cell Selection

Traceless Affinity Cell Selection


Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection

  • High purity, yield and viability
  • Label-free cells
  • Directly from whole blood
  • No centrifugation

Fab-TACS™ Gravity and FABian™ »

Magnetic Cell Selection


Magnetic Cell Selection

IBA's Streptamer™ »

  • Fully reversible
  • No stimulation of cells after cell separation
  • Optimal preservation of cell effector function 
  • Improved viability of cells 

Cell Stimulation & Expansion


Cell Stimulation & Expansion

CD3/CD28 Streptamers™ »

  • Completely reversible reagents
  • Detachment from the cells at any given point in time
  • Adjustable CD3 : CD28 ratio 
  • Non-magnetic and bead-free

Peptides for T cell stimulation »

  • Purity > 80 %
  • Endotoxin free

Fluorescent Cell Staining


Fluorescent Cell Staining

Detection of antigen-specific T cells with MHC I Streptamers™ »

Detection of receptor-specific T cells with Fab Streptamers™ »

  • Fully reversible
  • High specificity, sensitivity and reproducibility
  • No leftover of staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Protein
Protein

Cloning & Transfection


Cloning & Transfection

More than 150 expression vectors »

  • Variety of affinity tags
  • Expression hosts:  E. coli, mammalia, insect cells, yeast
  • StarGate cloning system

Mammalian Expression


The MEXi system »

Optimized for efficient protein expression and Strep-tag™ purification in mammalian cells.

Protein Purification


Protein Purification

Strep-tag™ system from the original manufacturer

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Detection


Detection

For Western blot analysis, immunofluorescence, FACS and ELISA »

  • Strep-Tactin™ conjugates for Strep-tag™II/Twin-Strep-tag™ proteins
  • Monoclonal antibodies against Strep-tag™II/Twin-Strep-tag™

Immobilisation


Immobilisation & Immunoprecipitation

Near covalent binding affinity – Immobilisation by Strep-Tactin™XT:Twin-Strep-tag™ for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore)

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated

Immunoprecipitation


Selector resins based on high-affinity single-domain antibodies (sdAb)

  • High capacity
  • Minimal unspecific binding
Oligos
13473_IBA_oligo_Banner

DNA


RNA


DNA & RNA

Standard DNA oligonucleotides

  • Long range up to 200 bases*
  • Large scale oligonucleotides
  • 3’-, 5’- or internally modified

Random RNA, dsRNA, siRNA, miRNA

  • Long range up to 100 bases*
  • 3’-, 5’- or internally modified
  • 2‘-modified: -O-Me, -F, -NH2
  • Nucleoside Triphosphates
  • RNA and RNA/DNA hybrid
  • Dinucleotides

*depending on sequence and scale

Fluorescence Labels


Non-Fluorescence Labels


Fluorescence Labels

E.g. for FRET, qPCR, FCS/FCCS, confocal and STED microscopy, single-molecule imaging, multi-color DNA and RNA FISH, DNA Origami

  • For dual and multiply labeled DNA and RNA
  • Excellent high quality 
  • More than 200 labels to choose from
  • Quenchers such as BBQ and BHQ™
  • Completely new dye combinations available

For DNA microarrays, surface binding and hybridization experiments, cross-linking and crystallography studies and more

  • More than 80 internal, 5’- and 3’- DNA and RNA modifications 

Backbone Modifications


Aptamers


Backbone Modifications
  • Phosphorothioates

Custom synthesis of DNA and RNA aptamers

  • High affinity 
  • Low immunogenicity
Resources Strep-Tactin®XT Sample Request
IBA_about_us

About IBA GmbH

Solutions For Life Sciences

IBA GmbH provides innovative technologies for life science applications. The product portfolio includes tools for cell selection & expansion, protein production & assays as well as custom oligos & predefined DNA/RNA.