IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression

Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins.

Product Code	Brand	Description	Protein Tag
15633758	IBA Lifesciences 5-4200-001	pPSG-IBA wt1	Untagged
15643758	IBA Lifesciences 5-4203-001	pPSG-IBA 3	Strep-tag for C-terminal
15653758	IBA Lifesciences 5-4205-001	pPSG-IBA 5	Strep-tag for N-terminal
15663758	IBA Lifesciences 5-4223-001	pPSG-IBA 23	Strep-tag for C-terminal and GST-tag for N-terminal
15673758	IBA Lifesciences 5-4225-001	pPSG-IBA 25	GST Tag for N-terminal
15683758	IBA Lifesciences 5-4233-001	pPSG-IBA 33	His Tag for C-terminal
15693758	IBA Lifesciences 5-4235-001	pPSG-IBA 35	His Tag (6x) for N-terminal
15603768	IBA Lifesciences 5-4243-001	pPSG-IBA 43	Strep-tag for C-terminal and His Tag (6x) for N-terminal
15613768	IBA Lifesciences 5-4245-001	pPSG-IBA 45	Strep-tag for N-terminal and His Tag (6x) for C-terminal
15623768	IBA Lifesciences 5-4262-001	pPSG-IBA 62	Strep-tag for C-terminal and FLAG-tag for N-terminal
15633768	IBA Lifesciences 5-4263-001	pPSG-IBA 63	FLAG-tag for C-terminal
15643768	IBA Lifesciences 5-4264-001	pPSG-IBA 64	Strep-tag for N-terminal and FLAG-tag for C-terminal
15653768	IBA Lifesciences 5-4265-001	pPSG-IBA 65	FLAG-tag for N-terminal
15663768	IBA Lifesciences 5-4303-001	pPSG-IBA 103	Twin-Strep-tag for C-terminal
15673768	IBA Lifesciences 5-4305-001	pPSG-IBA 105	Twin-Strep-tag for N-terminal
15683768	IBA Lifesciences 5-4323-001	pPSG-IBA 123	Twin-Strep-tag for C-terminal and GST-tag for N-terminal
15693768	IBA Lifesciences 5-4343-001	pPSG-IBA 143	Twin-Strep-tag for C-terminal and His Tag (6x) for N-terminal
15603778	IBA Lifesciences 5-4345-001	pPSG-IBA 145	Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal
15613778	IBA Lifesciences 5-4362-001	pPSG-IBA 162	Twin-Strep-tag for C-terminal and FLAG-tag for N-terminal
15623778	IBA Lifesciences 5-4364-001	pPSG-IBA 164	Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal
15633778	IBA Lifesciences 5-4367-001	pPSG-IBA 167	Twin-Strep-tag/FLAG-tag for N-terminal
15643778	IBA Lifesciences 5-4368-001	pPSG-IBA 168	Twin-Strep-tag/FLAG-tag for C-terminal

Description

pPSG-IGA E. coli expression vectors:

pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest.

As the T7 promoter is stronger than the tet promoter, pPSG-IBA vectors are recommended in cases where expression with the tet promoter does not lead to significant yields of the recombinant protein.

• High-level expression in E. coli by bacteriophage T7 promoter
• High-level transcription by T7 RNA polymerase in BL21 strains
• High-level expression of non-toxic proteins
• Induction by IPTG
• Suitable for in vitro transcription/translation

StarGate System

IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

• Minimal extra modification of the gene due to short combinatorial sites
• Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
• GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
• Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
• The final expression vector, the Destination Vector, is then placed into the respective host

Specifications

• Format: Liquid
• Inducing Agent: E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3))
• Promoter: T7
• Storage Buffer: 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
• Concentration: 250 ng/μL
• Vector: Total length: 3469
• For Use With (Application): Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins

• For Use With (Equipment): StarGate™ System, E. Coli BL21(DE3)
• pH: 8
• Product Type: Acceptor Vector for E. coli expression
• Content And Storage: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Quantity: 5 μg
• Color: Clear

Safety and Handling

missing translation for 'shelfLife' : 12 months