IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA Lifesciences GmbH Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).

Product Code	Brand	Vector
15613858	IBA Lifesciences 5-5003-001	pCSG-IBA3
15613868	IBA Lifesciences 5-5064-001	pCSG-IBA64
15613878	IBA Lifesciences 5-5145-001	pCSG-IBA145
15623858	IBA Lifesciences 5-5005-001	pCSG-IBA5
15623878	IBA Lifesciences 5-5162-001	pCSG-IBA162
15633858	IBA Lifesciences 5-5023-001	pCSG-IBA23
15633878	IBA Lifesciences 5-5164-001	pCSG-IBA164
15643868	IBA Lifesciences 5-5103-001	pCSG-IBA103
15643878	IBA Lifesciences 5-5167-001	pCSG-IBA167
15653878	IBA Lifesciences 5-5168-001	pCSG-IBA168
15663868	IBA Lifesciences 5-5105-001	pCSG-IBA105
15673858	IBA Lifesciences 5-5043-001	pCSG-IBA43
15673868	IBA Lifesciences 5-5123-001	pCSG-IBA123
15683858	IBA Lifesciences 5-5045-001	pCSG-IBA45
15693848	IBA Lifesciences 5-5000-001	pCSG-IBAwt1
15693858	IBA Lifesciences 5-5062-001	pCSG-IBA62
15693868	IBA Lifesciences 5-5143-001	pCSG-IBA143

Description

pCSG-IBA mammalian expression vectors

• Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
  • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
  • Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
• Stable cell lines can be selected by the neomycin resistance gene (NeoR).
• In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.

Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.

Specifications

• Constitutive or Inducible System: Constitutive
• Promoter: CMV
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Cloning Method: Direct Cloning
• Primer: Forward
• For Use With (Application): Episomal expression (oriP, EBNA-1)

• Marker Gene: Ampicillin
• Product Type: Vector
• Content And Storage: Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Concentration: 250 ng/μL
• Quantity: 5 μg