Dissociation Reagents
Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)
A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells
Corning™ Matrigel™ Membrane Matrix
Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
Gibco™ MEM Non-Essential Amino Acids Solution (100X)
Used as a growth supplement for cell culture medium, to increase cell growth and viability
Invitrogen™ Tryptose Phosphate Broth
Cell culture supplement for BHK-21 cells used for vaccine production
Gibco™ GlutaMAX™ Supplement
An alternative to L-glutamine, with increased stability that improves cell health
Corning™ Cell-Tak Cell and Tissue Adhesive
Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis.
Gibco™ L-Glutamine (200 mM)
Ready-to-use stock solution that acts as a cell culture supplement
Corning™ Cell Recovery Solution
Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses
Gibco™ Trypan Blue Solution, 0.4%
Routinely used as a cell stain to assess cell viability using the dye exclusion test
Gibco™ ACK Lysing Buffer
Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow
Gibco™ Pluronic™ F-68 Non-ionic Surfactant (100X)
A non-ionic surfactant used to control shear forces in suspension cultures
Corning™ Matrigel™ GFR Membrane Matrix
Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
Gibco™ Gurr Buffer Tablets
A convenient way to prepare a pH 6.8 phosphate buffer for dilution of Giemsa stain
Gibco™ Sodium Pyruvate (100 mM)
Growth supplement
Gibco™ Phytohemagglutinin, M form (PHA-M)
This extract is capable of inducing blastogenesis in vitro in various mammalian mononuclear cells when used at a concentration of 1 to 2% v/v
Gibco™ 2-Mercaptoethanol
A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals
Corning™ Fibronectin, Human
Promotes cellular attachment, spreading, and proliferation on tissue-culture surfaces. Corning™ Fibronectin, Human can be used as an additive to serum-free culture media.
Corning™ Matrigel™ hESC-Qualified Matrix
Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.
MP Biomedicals™ Poly-L-Lysine Hydrobromide Attachment Factors: 30,000-70,000 MW
Facilitate attachment, adherence, and spreading in the culture of adherent cells
Gibco™ Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X)
A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells
Gibco™ HAT Supplement (50X)
HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells
Invitrogen™ Zymosan A S. cerevisiae BioParticles™, unlabeled
Nonfluorescent products are useful in phagocytosis assays, both as controls and for custom labeling with reactive dyes
Invitrogen™ Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 488 Streptavidin (Alexa Fluor™ 488 EGF complex)
The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe affinity for the EGF receptor
Invitrogen™ Low Density Lipoprotein from Human Plasma, Acetylated, DiI complex (DiI AcLDL)
Acetylated (Ac) apoprotein used to study cell types that express receptors specific for this acetylated version, i.e., endothelial and microglial cells
Corning™ PuraMatrix™ Peptide Hydrogel
Highly defined synthetic peptide assembles into fibrous 3D structure and promotes cell attachment
Gibco™ MEM Amino Acids Solution (50X)
Used as a growth supplement for cell culture medium, to increase cell growth and viability
MP Biomedicals™ Poly-L-Lysine Hydrobromide Attachment Factors: ≥70,000 MW
Facilitate attachment, adherence, and spreading in the culture of adherent cells
Gibco™ KaryoMAX™ Giemsa Stain Solution
For G-banding of chromosomes for cytogenetic analysis
Gibco™ HT Supplement (100X)
HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.