Electrophoresis, Western Blotting and ELISA

Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Thermo Scientific™ PageRuler™ Prestained Protein Ladder, 10 to 180 kDa

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Invitrogen™ Mark12™ Unstained Standard

Allows the closest estimation of molecular weight of your sample protein over a wide molecular weight range

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

Invitrogen™ SeeBlue™ Plus2 Pre-stained Protein Standard

Allows you to easily visualize protein molecular weight ranges during electrophoresis and quickly evaluate Western transfer efficiency

Invitrogen™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Invitrogen™ SimplyBlue™ SafeStain

Less hazardous and does not require methanol or acetic acid fixatives or destains

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Invitrogen™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Invitrogen™ Novex™ Sharp Pre-stained Protein Standard

Designed for accurate, easy, and convenient molecular weight estimation of a wide range of molecular weight proteins during SDS-PAGE and Western blotting

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Thermo Scientific™ 1-Step™ Ultra TMB-ELISA Substrate Solution

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Invitrogen™ Colloidal Blue Staining Kit

Five times more sensitivity than Coomassie™ R-250— and can detect as little as 10ng of BSA

Thermo Scientific™ Spectra™ Multicolor Broad Range Protein Ladder

A 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use as size standards in gel electrophoresis and Western blotting.

Invitrogen™ NativePAGE™ 4-16% Bis-Tris Protein Gels, 1.0 mm, 10-well

Separation Range: 30 to 10,000 kDa; Separation Type: Native; For Use With: Mini Gel Tank, XCell SureLock Mini

Thermo Scientific™ PageRuler™ Unstained Low Range Protein Ladder

A mixture of seven purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis.

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Invitrogen™ NuPAGE™ 4-12% Bis-Tris Midi Protein Gels, 20-well

Separation Range: 3.5 to 260 kd; Separation Type: Denaturing (MES Running Buffer, MOPS Buffer); For Use With: Midi Gel Tank, XCell4 SureLock Midi

Thermo Scientific™ Pierce™ Lane Marker Non-Reducing Sample Buffer

Ready-to-use 5X SDS-PAGE sample loading buffers available in reducing and nonreducing formulations, both with a pink dye buffer-front marker.

Invitrogen™ SeeBlue™ Pre-stained Protein Standard

Allows you to easily monitor the progress of your gel run and is an excellent tool for judging Western transfer efficiency

Invitrogen™ iBlot™ Transfer Stack, nitrocellulose, mini

iBlot Transfer Stacks are used to transfer proteins

Thermo Scientific™ Sealing Tape for 96-Well Plates

Clear, self-sticking sealing tape for standard microplates.

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