IBA GmbH
Twin-Strep-tag™/Strep-Tactin™XT
![13473_IBA_strep-tactinXT Twin-Strep-tag](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab1/columncontrol_644a/colPar1/imageandrte_5ebd.img.jpg/1571407294196.jpg)
The purification system with the highest affinity – one tag for expression, purification, detection and immobilization
- High affinity in low pM range
- Rapid one-step purification with unparalleled protein purity (> 95 %)
- Mild and specific elution
- Tolerates broad range of buffer conditions
- Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other
Immobilization with near covalent binding affinity
![13473_IBA_109_rz-1 Immobilization with near covalent binding affinity](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab1/columncontrol_c171/colPar1/imageandrte_5ebd.img.jpg/1571407294213.jpg)
Twin-Strep-tag™ Capture Kit for SPR experiments »
- High affinity in low pM range enables long term measurements (T1/2 = 13 h)
- Chip can be easily regenerated
High affinity meets high capacity!
![13473_IBA_STXT-hc-products3 High affinity meets high capacity!](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab1/columncontrol_394c/colPar1/imageandrte_5ebd.img.jpg/1571407294231.jpg)
Strep-Tactin™XT Superflow™ high capacity »
- Higher protein yields compared to all other Strep-Tactin™ resins
- Dynamic binding capacity of ~15 mg protein per ml resin
- Elution of concentrated protein due to small elution volumes
- Cost efficent: small price for more protein
Try our new high affinity Strep-tag™ system
![13473_IBA_2-4998-000 Try our new high affinity Strep-tag® system](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab1/columncontrol_f3cd/colPar1/imageandrte_5ebd.img.jpg/1571407294248.jpg)
Starter Kit consists of:
- Strep-Tactin™XT Superflow™ gravity flow column
- Buffer W (Strep-Tactin™/Strep-Tactin™XT Wash Buffer)
- Buffer BXT (Strep-Tactin™XT Elution Buffer with Biotin)
- Strep-Tactin™ conjugated with HRP (for Western blot detection)
- Short protocol
Traceless Affinity Cell Selection
![13473_IBA_244 Traceless Affinity Cell Selection](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab2/columncontrol_d058/colPar1/imageandrte_d70a.img.jpg/1571407294096.jpg)
Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection
- High purity, yield and viability
- Label-free cells
- Directly from whole blood
- No centrifugation
Magnetic Cell Selection
![13473_IBA_066_rz-1 Magnetic Cell Selection](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab2/columncontrol_626/colPar1/imageandrte_d70a.img.jpg/1571407294114.jpg)
- Fully reversible
- No stimulation of cells after cell separation
- Optimal preservation of cell effector function
- Improved viability of cells
Cell Stimulation & Expansion
![13473_IBA_095_rz-1 Cell Stimulation & Expansion](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab2/columncontrol_cc81/colPar1/imageandrte_d70a.img.jpg/1571407294146.jpg)
- Completely reversible reagents
- Detachment from the cells at any given point in time
- Adjustable CD3 : CD28 ratio
- Non-magnetic and bead-free
Peptides for T cell stimulation »
- Purity > 80 %
- Endotoxin free
Fluorescent Cell Staining
![13473_IBA_099_rz-1 Fluorescent Cell Staining](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab2/columncontrol_c967/colPar1/imageandrte_d70a.img.jpg/1571407294163.jpg)
Detection of antigen-specific T cells with MHC I Streptamers™ »
Detection of receptor-specific T cells with Fab Streptamers™ »
- Fully reversible
- High specificity, sensitivity and reproducibility
- No leftover of staining reagents on the cells after FACS sorting
- Optimal preservation of cell effector function
- Improved viability of cells
Cloning & Transfection
![13473_IBA_106_rz-1 Cloning & Transfection](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab3/columncontrol_40ad/colPar1/imageandrte_b27a.img.jpg/1571407293990.jpg)
More than 150 expression vectors »
- Variety of affinity tags
- Expression hosts: E. coli, mammalia, insect cells, yeast
- StarGate cloning system
Mammalian Expression
Optimized for efficient protein expression and Strep-tag™ purification in mammalian cells.
Protein Purification
![13473_IBA_078_rz-1 Protein Purification](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab3/columncontrol_ef5d/colPar1/imageandrte_d70a.img.jpg/1571407294023.jpg)
Strep-tag™ system from the original manufacturer
- High affinity in low pM range
- Rapid one-step purification with unparalleled protein purity (> 95 %)
- Mild and specific elution
- Tolerates broad range of buffer conditions
- Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other
Detection
![13473_IBA_102_rz-1 Detection](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab3/columncontrol_7919/colPar1/imageandrte_d70a.img.jpg/1571407294040.jpg)
For Western blot analysis, immunofluorescence, FACS and ELISA »
- Strep-Tactin™ conjugates for Strep-tag™II/Twin-Strep-tag™ proteins
- Monoclonal antibodies against Strep-tag™II/Twin-Strep-tag™
Immobilisation
![13473_IBA_109_rz-1 Immobilisation & Immunoprecipitation](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab3/columncontrol_2cad/colPar1/imageandrte_2ed9.img.jpg/1571407294051.jpg)
Near covalent binding affinity – Immobilisation by Strep-Tactin™XT:Twin-Strep-tag™ for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore)
- High affinity in low pM range enables measurements over a long time
- Minimal non-specific binding
- Minimal coefficients of variation (cv)
- Chip for SPR experiments can be easily regenerated
Immunoprecipitation
Selector resins based on high-affinity single-domain antibodies (sdAb)
- High capacity
- Minimal unspecific binding
DNA
RNA
![13473_IBA_059_rz-1 DNA & RNA](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab4/columncontrol_5efd/colPar1/imageandrte_1479.img.jpg/1571407293848.jpg)
Standard DNA oligonucleotides
- Long range up to 200 bases*
- Large scale oligonucleotides
- 3’-, 5’- or internally modified
Random RNA, dsRNA, siRNA, miRNA
- Long range up to 100 bases*
- 3’-, 5’- or internally modified
- 2‘-modified: -O-Me, -F, -NH2
- Nucleoside Triphosphates
- RNA and RNA/DNA hybrid
- Dinucleotides
*depending on sequence and scale
Fluorescence Labels
Non-Fluorescence Labels
![13473_IBA_082_rz-1 Fluorescence Labels](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab4/columncontrol_90c8/colPar1/imageandrte_1479.img.jpg/1571407293891.jpg)
E.g. for FRET, qPCR, FCS/FCCS, confocal and STED microscopy, single-molecule imaging, multi-color DNA and RNA FISH, DNA Origami
- For dual and multiply labeled DNA and RNA
- Excellent high quality
- More than 200 labels to choose from
- Quenchers such as BBQ and BHQ™
- Completely new dye combinations available
For DNA microarrays, surface binding and hybridization experiments, cross-linking and crystallography studies and more
- More than 80 internal, 5’- and 3’- DNA and RNA modifications
Backbone Modifications
Aptamers
![13473_IBA_218_rz-1 Backbone Modifications](/be/fr/brands/i/iba/jcr%3acontent/silver_page/silvertabs_82ab/silverTab4/columncontrol_d4a8/colPar1/imageandrte_1479.img.jpg/1571407293934.jpg)
- Phosphorothioates
Custom synthesis of DNA and RNA aptamers
- High affinity
- Low immunogenicity
Cell Selection & Expansion
- Application Note FABian Lymphocyte Isolation
- Application Note Central memory T-cells isolated and stained with CD8α and CD62L Fab Streptamer reagents
- Application Note Combining MHC-I and CD8+ Fab Streptamer reagents
- Application Note Regulatory T-cells isolated and stained with CD4-CD25 and CD45RA Fab Streptamer reagents
- Application Note CD3-T Lymphocytes isolated and stained with Streptamer reagents
Custom Oligos & Predefined DNA/RNA
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About IBA GmbH
Solutions For Life Sciences
IBA GmbH provides innovative technologies for life science applications. The product portfolio includes tools for cell selection & expansion, protein production & assays as well as custom oligos & predefined DNA/RNA.