IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression

Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.

Product Code	Brand	Description	Protein Tag
15613808	IBA Lifesciences 5-4600-001	pYSG-IBA wt1	Untagged
15623808	IBA Lifesciences 5-4603-001	pYSG-IBA 3	Strep-tag for C-terminal
15633808	IBA Lifesciences 5-4605-001	pYSG-IBA 5	Strep-tag for N-terminal
15643808	IBA Lifesciences 5-4623-001	pYSG-IBA 23	Strep-tag for C-terminal and GST-tag for N-terminal
15653808	IBA Lifesciences 5-4625-001	pYSG-IBA 25	GST Tag for N-terminal
15663808	IBA Lifesciences 5-4633-001	pYSG-IBA 33	His Tag (6x) for C-terminal
15673808	IBA Lifesciences 5-4643-001	pYSG-IBA 43	His Tag (6x) for N-terminal and Strep-tag for C-terminal
15683808	IBA Lifesciences 5-4645-001	pYSG-IBA 45	Strep-tag for N-terminal and His Tag (6x) for C-terminal
15693808	IBA Lifesciences 5-4662-001	pYSG-IBA 62	Strep-tag for C-terminal and FLAG-tag for N-terminal
15603818	IBA Lifesciences 5-4663-001	pYSG-IBA 63	FLAG-tag for C-terminal
15613818	IBA Lifesciences 5-4664-001	pYSG-IBA 64	Strep-tag for N-terminal and FLAG-tag for C-terminal
15623818	IBA Lifesciences 5-4665-001	pYSG-IBA 65	FLAG-tag for N-terminal
15633818	IBA Lifesciences 5-4703-001	pYSG-IBA 103	Twin-Strep-tag for C-terminal
15643818	IBA Lifesciences 5-4705-001	pYSG-IBA 105	Twin-Strep-tag for N-terminal
15653818	IBA Lifesciences 5-4723-001	pYSG-IBA 123	Twin-Strep-tag for C-terminal and GST-tag for N-terminal
15663818	IBA Lifesciences 5-4743-001	pYSG-IBA 143	His Tag (6x) for N-terminal and Twin-Strep-tag for C-terminal
15673818	IBA Lifesciences 5-4745-001	pYSG-IBA 145	Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal
15683818	IBA Lifesciences 5-4762-001	pYSG-IBA 162	Twin-Strep-tag for C-terminal and FLAG-tag for N-terminal
15693818	IBA Lifesciences 5-4764-001	pYSG-IBA 164	Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal
15603828	IBA Lifesciences 5-4767-001	pYSG-IBA 167	Twin-Strep-tag/FLAG-tag for N-terminal
15613828	IBA Lifesciences 5-4768-001	pYSG-IBA 168	Twin-Strep-tag/FLAG-tag for C-terminal

Description

pYSG-IBA yeast expression vectors

The pYSG-IBA vector allows the high-level expression of proteins in yeast.

• The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
• The expressed recombinant protein will be localized in the cytoplasm.

 

• High-level expression in yeast with high copy number
• Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
• Tightly regulated expression due to Cu⁺⁺-inducible CUP1 promoter
• Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
• Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
• URA3 auxotrophy marker for selection after transformation

Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

• Format: Liquid
• Inducing Agent: Copper sulfate
• pH: 8
• Product Type: Acceptor Vector for yeast expression
• Content And Storage: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Quantity: 5 μg
• For Use With (Application): Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins

• For Use With (Equipment): StarGate™ System, Yeast Host Cells
• Marker Gene: leu2-d and URA3
• Promoter: CUP1
• Storage Buffer: 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
• Concentration: 250 ng/μL
• Color: Clear

Safety and Handling

missing translation for 'shelfLife' : 12 months