Thermo Scientific Chemicals Trypan Blue, dye content >60%

Product Code	Brand	Quantity
11458517	Thermo Scientific Chemicals A18600.14	25 g
11468517	Thermo Scientific Chemicals A18600.22	100 g
11478517	Thermo Scientific Chemicals A18600.36	500 g

Description

Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell cluster cell viability from caprine pancreatic samples. It is also useful to observe hyphea of fungi and Stramenopiles. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell cluster cell viability from caprine pancreatic samples. It is also useful to observe hyphea of fungi and Stramenopiles. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.

Solubility
Soluble in water (10 mg/ml at 25°C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).

Notes
Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Chemical Identifiers

• CAS: 72-57-1
• Molecular Weight (g/mol): 960.79
• InChI Key: WMYVHJWZUUEZNE-ARWFNKCKSA-J
• IUPAC Name: tetrasodium;(3Z)-5-amino-3-[[4-[4-[(2Z)-2-(8-amino-1-oxo-3,6-disulfonatonaphthalen-2-ylidene)hydrazinyl]-3-methylphenyl]-2-methylphenyl]hydrazinylidene]-4-oxonaphthalene-2,7-disulfonate

• Molecular Formula: C34H24N6Na4O14S4
• MDL Number: MFCD00003969
• PubChem CID: 9562061
• SMILES: [Na+].[Na+].[Na+].[Na+].CC1=CC(=CC=C1N\N=C1\C(=O)C2=C(N)C=C(C=C2C=C1S([O-])(=O)=O)S([O-])(=O)=O)C1=CC=C(N\N=C2\C(=O)C3=C(N)C=C(C=C3C=C2S([O-])(=O)=O)S([O-])(=O)=O)C(C)=C1

Specifications

• Melting Point: >300°C
• Molecular Formula: C34H24N6Na4O14S4
• Merck Index: 14,9792
• InChI Key: WMYVHJWZUUEZNE-ARWFNKCKSA-J
• IUPAC Name: tetrasodium;(3Z)-5-amino-3-[[4-[4-[(2Z)-2-(8-amino-1-oxo-3,6-disulfonatonaphthalen-2-ylidene)hydrazinyl]-3-methylphenyl]-2-methylphenyl]hydrazinylidene]-4-oxonaphthalene-2,7-disulfonate
• PubChem CID: 9562061
• Chemical Name or Material: Trypan Blue, dye content >60%

• CAS: 72-57-1
• MDL Number: MFCD00003969
• Solubility Information: Soluble in water (10mg/ml at 25°C),ethanol (0.6mg/ml),and EGME (20mg/ml).
• SMILES: [Na+].[Na+].[Na+].[Na+].CC1=CC(=CC=C1N\N=C1\C(=O)C2=C(N)C=C(C=C2C=C1S([O-])(=O)=O)S([O-])(=O)=O)C1=CC=C(N\N=C2\C(=O)C3=C(N)C=C(C=C3C=C2S([O-])(=O)=O)S([O-])(=O)=O)C(C)=C1
• Molecular Weight (g/mol): 960.79
• Formula Weight: 960.82

Safety and Handling

GHS H Statement
H350

P201-P202-P281-P308+P313-P501c

H350

missing translation for 'einecsNumber' : 200-786-7

missing translation for 'rtecsNumber' : QJ6475000

missing translation for 'tsca' : Yes

Recommended Storage : Ambient temperatures

Product Identifier

• Trypan Blue

Signal Word

• Danger

Hazard Category

• Carcinogenicity Category 1B

Hazard Statement

• H350-May cause cancer.

Precautionary Statement

• P201-Obtain special instructions before use.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P308+P313-IF exposed or concerned: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : Trypan Blue

RUO – Research Use Only