Thermo Scientific Chemicals Trypan Blue, dye content >60%

Description

Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell cluster cell viability from caprine pancreatic samples. It is also useful to observe hyphea of fungi and Stramenopiles. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell cluster cell viability from caprine pancreatic samples. It is also useful to observe hyphea of fungi and Stramenopiles. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.

Solubility
Soluble in water (10 mg/ml at 25°C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).

Notes
Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Specifications

Chemical Identifiers

• CAS: 72-57-1
• Molecular Formula: C34H24N6Na4O14S4
• Molecular Weight (g/mol): 960.79
• MDL Number: MFCD00003969
• InChI Key: WMYVHJWZUUEZNE-ARWFNKCKSA-J
• Synonym: C.I. 23850; Direct Blue 14
• PubChem CID: 9562061
• IUPAC Name: tetrasodium;(3Z)-5-amino-3-[[4-[4-[(2Z)-2-(8-amino-1-oxo-3,6-disulfonatonaphthalen-2-ylidene)hydrazinyl]-3-methylphenyl]-2-methylphenyl]hydrazinylidene]-4-oxonaphthalene-2,7-disulfonate
• SMILES: [Na+].[Na+].[Na+].[Na+].CC1=CC(=CC=C1N\N=C1\C(=O)C2=C(N)C=C(C=C2C=C1S([O-])(=O)=O)S([O-])(=O)=O)C1=CC=C(N\N=C2\C(=O)C3=C(N)C=C(C=C3C=C2S([O-])(=O)=O)S([O-])(=O)=O)C(C)=C1

• Melting Point: >300°C
• Merck Index: 14,9792
• Solubility Information: Soluble in water (10mg/ml at 25°C),ethanol (0.6mg/ml),and EGME (20mg/ml).
• Formula Weight: 960.82
• Quantity: 100 g
• Chemical Name or Material: Trypan Blue, dye content >60%

RUO – Research Use Only