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Invitrogen™ NOTCH-1 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHNOTCH1

Code : NEW

Additional Details : Weight : 0.33000kg

Product Code. 15455083

  • £452.00 / 1 set


Estimated Shipment: 05-07-2021
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Description

Description

The Human NOTCH-1 ELISA quantitates Hu NOTCH-1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NOTCH-1. Principle of the method The Human NOTCH-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).
Specifications

Specifications

P46531
ELISA Kit
Biotin
28.67-7000 pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 50 μL|Serum, 50 μL|Supernatant, 100 μL
2°C to 8°C
1 hr. 20 min.
Human
AOS5, AOVD1, TAN1, hN1
<10%
Human
20 pg/mL
Colorimetric Microplate Reader
HRP
Solid-phase sandwich ELISA
Chemokines & Receptors
20pg/mL to 7000pg/mL
4851
RUO
The sandwich ELISA antibody pair detects Human NOTCH-1.
96 assays
4 hr. 45 min.
Angiogenesis
<12%
96 Tests
Supernatant
AOS5,AOVD1,TAN1,hN1
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