Invitrogen™ NOTCH-1 Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15455083	Invitrogen™ EHNOTCH1	96 Tests
17185519	Invitrogen™ EHNOTCH1X5	5 x 96 Tests

Description

The Human NOTCH-1 ELISA quantitates Hu NOTCH-1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NOTCH-1. Principle of the method The Human NOTCH-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

Specifications

• Accession Number: P46531
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Specificity: The sandwich ELISA antibody pair detects Human NOTCH-1.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 4851
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Angiogenesis
• Synonym: AOS5, AOVD1, TAN1, hN1
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 20 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: AOS5,AOVD1,TAN1,hN1
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.