IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA Lifesciences GmbH E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli.

Product Code	Brand	Vector
15603758	IBA Lifesciences 5-4164-001	pASG-IBA164
15613758	IBA Lifesciences 5-4167-001	pASG-IBA167
15623748	IBA Lifesciences 5-4103-001	pASG-IBA103
15623758	IBA Lifesciences 5-4168-001	pASG-IBA168
15633728	IBA Lifesciences 5-4000-001	pASG-IBAwt1
15633738	IBA Lifesciences 5-4043-001	pASG-IBA43
15643748	IBA Lifesciences 5-4105-001	pASG-IBA105
15653738	IBA Lifesciences 5-4045-001	pASG-IBA45
15653748	IBA Lifesciences 5-4123-001	pASG-IBA123
15663728	IBA Lifesciences 5-4003-001	pASG-IBA3
15663738	IBA Lifesciences 5-4062-001	pASG-IBA62
15663748	IBA Lifesciences 5-4143-001	pASG-IBA143
15683728	IBA Lifesciences 5-4005-001	pASG-IBA5
15683738	IBA Lifesciences 5-4064-001	pASG-IBA64
15683748	IBA Lifesciences 5-4145-001	pASG-IBA145
15693728	IBA Lifesciences 5-4023-001	pASG-IBA23
15693748	IBA Lifesciences 5-4162-001	pASG-IBA162

Description

• The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.
• The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.
• The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.
• Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.

Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

• Inducing Agent: Inducible with anhydrotetracycline
• Promoter: Tet
• Content And Storage: Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Quantity: 5 μg

• Marker Gene: Ampicillin
• Product Type: Vector
• Concentration: 250 ng/μL