IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA Lifesciences GmbH E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli.

Marque:  IBA Lifesciences 5-4105-001

Code produit. 15643748

Description

• The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.
• The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.
• The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.
• Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.

Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Spécification

• Inducing Agent: Inducible with anhydrotetracycline
• Promoter: Tet
• Content And Storage: Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Protein Tag: Twin-Strep-tag
• Concentration: 250 ng/μL
• Quantity: 5 μg
• For Use With (Application): Vector for recombinant expression in E. coli

• Marker Gene: Ampicillin
• Product Type: Vector
• Species: E.coli
• Cloning Method: Direct Cloning
• Primer: Forward
• Vector: pASG-IBA105