Protein Extraction and Purification

Cytiva Sephadex™ LH-20

Cytiva Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

Cytiva HiTrap™ Protein G HP Prepacked Columns

Cytiva HiTrap™ Protein G HP Prepacked Columns

Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ NAb™ Protein G Spin Kit, 0.2 mL

Thermo Scientific™ NAb™ Protein G Spin Kit, 0.2 mL

Affinity purify antibodies (IgG) with immobilized recombinant Protein G agarose offered in bottles, spin columns, filter plates and complete purification kits.

Cytiva GSTrap™ 4B Prepacked Columns: 7 × 25mm

Cytiva GSTrap™ 4B Prepacked Columns: 7 × 25mm

Use for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ 4B Prepacked Columns: 7 × 25mm are prepacked with Glutathione Sepharose™ 4B

MP Biomedicals™ Spin Filters

MP Biomedicals™ Spin Filters

For use in DNA Purification

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Invitrogen™ MagnaRack™ Magnetic Separation Rack

Invitrogen™ MagnaRack™ Magnetic Separation Rack

Holds up to 24 microcentrifuge tubes

Thermo Scientific™ PMSF Protease Inhibitor

Thermo Scientific™ PMSF Protease Inhibitor

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

Invitrogen™ DynaMag™-50 Magnet

Invitrogen™ DynaMag™-50 Magnet

Optimized for efficient cell isolation using Dynabeads™

Cytiva His SpinTrap™ Spin Columns and Kit

Cytiva His SpinTrap™ Spin Columns and Kit

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns and Kit include prepacked, single-use spin columns.

Thermo Scientific™ Pierce™ GST Spin Purification Kit, 1 mL

Thermo Scientific™ Pierce™ GST Spin Purification Kit, 1 mL

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads

Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads

Perform immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Cytiva His GraviTrap™ Columns

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

Invitrogen™ eBioscience™ 1X RBC Lysis Buffer

Invitrogen™ eBioscience™ 1X RBC Lysis Buffer

Formulated for optimal lysis of erythrocytes in single-cell suspensions of mouse hematopoietic tissues such as spleen and human peripheral blood.

Invitrogen™ Magnetic Stand-96

Invitrogen™ Magnetic Stand-96

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO

Thermo Scientific™ DNase I Solution (2500 U/mL)

Thermo Scientific™ DNase I Solution (2500 U/mL)

Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.

Thermo Scientific™ Sodium Deoxycholate Detergent

Thermo Scientific™ Sodium Deoxycholate Detergent

Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.

Cytiva Chelating Sepharose™ Fast Flow Medium

Cytiva Chelating Sepharose™ Fast Flow Medium

Use for purifying native and recombinant proteins and peptides that have an affinity for metal ions. Chelating Sepharose™ Fast Flow Medium provides robust performance and regulatory support.

Cytiva Sephadex™ Ion Exchange Media

Cytiva Sephadex™ Ion Exchange Media

Separate molecules over broad molecular weight and pH ranges

IBA Lifesciences Strep-Tactin™XT Spin Column Kit

IBA Lifesciences Strep-Tactin™XT Spin Column Kit

IBA Lifesciences GmbH Strep-Tactin™XT Spin Column Kit allows for rapid parallel purification of Strep-tag™ and Twin-Strep-tag™ fusion proteins.

Thermo Scientific™ Pierce™ Co-Immunoprecipitation Kit

Thermo Scientific™ Pierce™ Co-Immunoprecipitation Kit

Covalently immobilize IP antibodies and perform co-IP assays in which target protein complexes are immunoprecipitated without antibody contamination.

Thermo Scientific™ Protease Inhibitor Cocktails

Thermo Scientific™ Protease Inhibitor Cocktails

Protect your sample from proteolytic degradation during cell lysis with these broad-spectrum protease inhibitor cocktails.

Thermo Scientific™ GlycoLink™ IP Kit

Thermo Scientific™ GlycoLink™ IP Kit

Immobilize polyclonal antibodies or other glycoproteins onto agarose beads for immunoprecipitation or pull-down assays via periodate-oxidized sugars.

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