Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Brand:  Thermo Scientific™ 88224

Product Code. 12393730

Description

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.

The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.

Highlights:

• High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin

• Versatile – purify proteins using native or denaturing conditions

• Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives

• Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges

• Cost-effective – reuse the same batch of resin at least five times

• Easy to use – pre-formulated buffers available for kit formats

Available Formats:

• Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
• Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
• Purification Kits – complete kits in all three column sizes
• Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
• Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin

Includes:

Kits contain HisPur Ni-NTA Columns

Specifications

• Capacity (Metric): 0.2 mL
• Content And Storage: Store at 4°C
• Product Line: HisPur™
• Target: His-Tagged Protein
• Stationary Phase: Divalent Nickel, Metal Chelate
• Column Type: Affinity, Agarose Resin

• Description: HisPur Ni-NTA Spin Columns, 0.2mL
• Form: Liquid Suspension
• Quantity: 25 Columns
• Type: Spin Column
• Format: Microcentrifuge, Spin Column

Safety and Handling

Product Identifier

• HisPur Ni-NTA Spin Columns, 0.2 mL

Signal Word

• Danger

Hazard Category

• LONG-TERM AQUATIC HAZARD Chronic 3
• Carcinogenicity Category 1A
• Reproductive toxicity Category 1B
• Respiratory sensitiser Category 1
• Skin sensitiser Category 1
• Specific target organ toxicity after repeated exposure Category 2

Hazard Statement

• H317-May cause an allergic skin reaction.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H350-May cause cancer.
• H360D-May damage the unborn child.
• H373-May cause damage to organs.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Sulfuric acid, nickel(2+) salt, hydrate

For Research Use Only. Not for use in diagnostic procedures.