Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Product Code	Brand	Color	No. of Reactions
10024537	Thermo Scientific™ F530S	Colorless	100 Reactions
10287787	Thermo Scientific™ F530L	Colorless	500 Reactions
16254551	Thermo Scientific™ F-530XL	Colorless	2000 Reactions
13575160	Thermo Scientific™ F534S	Green	100 Reactions
13585160	Thermo Scientific™ F534L	Green	500 Reactions

Description

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features of Phusion™ High-Fidelity DNA Polymerase include:

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15–30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

Specifications

• GC-Rich PCR Performance: High
• Product Type: High-Fidelity DNA Polymerase
• For Use With (Application): Standard PCR, High-fidelity PCR
• Fidelity (vs. Taq): 52X
• Overhang: Blunt
• Size (Final Product): 20 kb or less

• Reaction Speed: Fast
• Shipping Condition: Dry Ice
• Concentration: 2 U/μL
• Hot Start: No
• Reaction Format: Standalone

For Research Use Only. Not for use in diagnostic procedures.