Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Brand:  Thermo Scientific™ F534L

Product Code. 13585160

Includes:

Phusion High-Fidelity DNA Polymerase

• Phusion DNA Polymerase 2 U/μL
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl₂ solution

Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl₂ in the final 1X concentration.

Phusion Green High-Fidelity DNA Polymerase

• Phusion DNA Polymerase 2 U/μL
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
• 50 mM MgCl₂ solution

Both Phusion Green HF Buffer and Phusion Green GC Buffer provide 1.5 mM MgCl₂ in the final 1X concentration.

Description

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features of Phusion™ High-Fidelity DNA Polymerase include:

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15–30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

Specifications

• Content And Storage: • Phusion DNA Polymerase, 2 U/μL
  • 5X Phusion Green HF and GF Buffers
  • DMSO
  • 50 mM MgCl₂
  
  Phusion Green HF and Phusion Green GC Buffers each provide 1.5 mM MgCl₂ in the final 1X concentration.
  Store at -20°C.
• Polymerase: Phusion High-Fidelity DNA Polymerase
• Product Type: High-Fidelity DNA Polymerase
• Shipping Condition: Dry Ice
• Concentration: 2 U/μL
• Hot Start: No
• Overhang: Blunt
• Size (Final Product): 20 kb or less

• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Quantity: 500 U
• For Use With (Application): Standard PCR, High-fidelity PCR
• Fidelity (vs. Taq): 52X
• No. of Reactions: 500 Reactions
• Reaction Format: Standalone
• Color: Green

For Research Use Only. Not for use in diagnostic procedures.