Invitrogen™ Osteopontin/SSP1 Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15455133	Invitrogen™ EHSPP1	96 Tests
17125569	Invitrogen™ EHSPP1X5	5 x 96 Tests

Description

The Human Osteopontin/SSP1 ELISA quantitates Hu SSP1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu SSP1. Principle of the method The Human SSP1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.

Specifications

• Accession Number: P10451
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 6696
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Synonym: PSEC0156, BNSP, BSPI, ETA-1, OPN
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 50 pg/mL
• Conjugate: Biotin
• Protein Family: Cell Structure & Organelle Proteins
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: BNSP,BSPI,ETA-1,OPN
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.