Invitrogen™ Osteopontin/SSP1 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHSPP1X5

Product Code. 17125569

Description

The Human Osteopontin/SSP1 ELISA quantitates Hu SSP1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu SSP1. Principle of the method The Human SSP1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.

Specifications

• Accession Number: P10451
• Assay Sensitivity: 50 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 6696
• Interassay CV: <12%
• Kit Contents: Chromogen, Pre-coated 96 well plates, Assay Diluent Concentrate, Stop Solution, Adhesive Plate Covers, Wash Buffer, Biotinylated Detection Antibody, SAV-HRP, Standard
• Quantity: 5 x 96 Tests
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 74.07 pg/mL to 18,000 pg/mL
• Calibration Range: 50 pg/mL to 18,000 pg/mL
• Conjugate: Biotin
• Protein Family: Cell Structure & Organelle Proteins
• Specificity: This ELISA kit shows no cross-reactivity with any of the following cytokines tested (human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.)
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: BNSP,BSPI,ETA-1,OPN
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 33 μ;L; Serum, 33 μ;L; Supernatant, 100 μ;L
• Synonym: PSEC0156, BNSP, BSPI, ETA-1, OPN
• Test Time: 1 hr. 20 min.