Cytiva Amersham™ AlkPhos Direct™ Labeling Modules

Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes. AlkPhos Direct Labeling Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase.

Brand:  Cytiva RPN3680

Product Code. 10298714

Description

DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.

• Hybridization stringency can be controlled by elevating temperatures up to 75°C
• Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
• Rapid protocol saves two to three hours over conventional indirect methods

Order Info

Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm² or 5000cm² membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent

Specifications

• Content And Storage: 2°C to 8°C
• For Use With (Application): Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes
• Product Type: AlkPhos Direct Labeling Module

• Description: AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star or ECF detection can be used.
• Includes: Labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent
• Quantity: 2500 cm2 Membrane

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• Skin sensitiser Category 1
• Germ cell mutagenicity Category 2
• Carcinogenicity Category 1B
• Specific target organ toxicity after single exposure Category 1

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H317-May cause an allergic skin reaction.
• H341-Suspected of causing genetic defects.
• H350-May cause cancer.
• H370-Causes damage to organs.

Precautionary Statement

• P201-Obtain special instructions before use.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : methanol, formaldehyde
• UNKNOWN ACUTE-100 per cent of the mixture consists of component(s) of unknown acute toxicity.
• UNKNOWN AQUATIC-Contains 100% of components with unknown hazards to the aquatic environment.

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