Cytiva Amersham™ AlkPhos Direct™ Labeling Modules

Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes. AlkPhos Direct Labeling Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase.

Product Code	Brand	Quantity
10298714	Cytiva RPN3680	2500 cm2 Membrane
10074344	Cytiva RPN3681	For 5000 cm2 membrane

Description

DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.

• Hybridization stringency can be controlled by elevating temperatures up to 75°C
• Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
• Rapid protocol saves two to three hours over conventional indirect methods

Order Info

Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm² or 5000cm² membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent

Specifications

• Content And Storage: 2°C to 8°C
• For Use With (Application): Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes
• Includes: Labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• Skin sensitiser Category 1
• Germ cell mutagenicity Category 2
• Carcinogenicity Category 1B
• Specific target organ toxicity after single exposure Category 1

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H317-May cause an allergic skin reaction.
• H341-Suspected of causing genetic defects.
• H350-May cause cancer.
• H370-Causes damage to organs.

Precautionary Statement

• P201-Obtain special instructions before use.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : methanol, formaldehyde
• UNKNOWN ACUTE-100 per cent of the mixture consists of component(s) of unknown acute toxicity.
• UNKNOWN AQUATIC-Contains 100% of components with unknown hazards to the aquatic environment.