Protein Extraction and Purification

Thermo Scientific™ Triton™ X-100 Surfact-Amps™ Detergent Solution

Thermo Scientific™ Surfact-Amps X-100 Detergent Solution is highly-purified Triton X-100 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific™ Sodium Deoxycholate Detergent

Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.

GE Healthcare Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. GE Healthcare Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells

Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit.

Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent

Extract total protein from tissue samples using this mild cell lysis and extraction reagent developed specifically for tissues.

Thermo Scientific™ Pepstatin A

Protease inhibitor, inhibits Pepsin, Renin and Cathepsin D.

Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

Invitrogen™ Magnetic-Ring Stand (96 well)

Designed for paramagnetic bead precipitation from standard 96-well U-bottom microplates

Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay.

Thermo Scientific™ HisPur™ Ni-NTA Spin Plates

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

GE Healthcare HiTrap™ Chelating HP Columns

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. GE Healthcare Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 1 mL

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Thermo Scientific™ PMSF Protease Inhibitor

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

Thermo Scientific™ RIPA Lysis and Extraction Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Thermo Scientific™ Pierce™ Streptavidin Plus UltraLink™ Resin

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ NP-40 Surfact-Amps™ Detergent Solution

Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ Pierce™ Protease Inhibitor Tablets, EDTA-free

Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit

Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.

Sartorius™ Vivaflow™ 50R, 200 Accessory Set

Complete set of accessories for Vivaflow 50R, 200 with 230V pump.

Thermo Scientific™ Brij™-35 Surfact-Amps™ Detergent Solution

Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.

Thermo Scientific™ Lysozyme

Degrade unwanted bacterial cell wall in protein or nucleic acid extracts

Thermo Scientific™ HiPPR™ Detergent Removal 96-well Spin Plates, 0.1 mL

Efficiently remove detergents from samples with this high protein and peptide recovery detergent removal resin, especially for dilute protein samples.

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