Buffers
Thermo Scientific™ Pierce™ 20X PBS Tween™ 20 Buffer
Prepare PBS-Tween (PBS-T) wash buffers and blocking buffers for ELISA and Western with this space-saving stock solution.
Thermo Scientific™ Pierce™ 10X Tris-Glycine SDS Buffer
Quickly prepare Tris-glycine-SDS (pH 8.3) running buffer for SDS-PAGE (protein polyacrylamide gel electrophoresis) with this space-saving stock solution.
Thermo Scientific™ BupH™ Carbonate-Bicarbonate Buffer Packs
Make 500mL of alkaline (pH 9.4) Na-carbonate (carb-bicarb) buffer for protein and antibody coating procedures with these dry-blended powder pouches.
Thermo Scientific™ BupH™ Phosphate Buffered Saline Packs
Make 500mL of sodium phosphate buffer (PBS) for crosslinking and labeling reactions requiring amine-free conditions with these dry-blended powder pouches.
Tris Hydrochloride, 1M Solution (pH 8.0/Mol. Biol.), Fisher BioReagents
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Tween™ 20, Fisher BioReagents™
CAS: 9005-64-5 Molecular Formula: C58H114O26 Molecular Weight (g/mol): 1227.54 InChI Key: HMFKFHLTUCJZJO-UHFFFAOYSA-N Synonym: Polysorbate 20, Polyoxyethylene-20-sorbitan Monolaurate, Polyoxyethylenesorbitan monolaurate PubChem CID: 443314 IUPAC Name: 2-[2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy]ethyl dodecanoate SMILES: CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane, Tris(hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents
Commonly used buffer
Buffer solution pH 7.0 (20°C), Potassium dihydrogen phosphate / disodium hydrogen phosphate, traceable to SRM from NIST, with fungicide, Honeywell Fluka™
CAS: 1336-21-6 Molecular Formula: H5NO Molecular Weight (g/mol): 35.046 MDL Number: MFCD00146206 InChI Key: VHUUQVKOLVNVRT-UHFFFAOYSA-N PubChem CID: 14923 ChEBI: CHEBI:18219 IUPAC Name: azanium;hydroxide SMILES: [NH4+].[OH-]
Buffer solution pH 10.0 (20°C), Violet colored, borax / sodium hydroxide, Honeywell Fluka™
CAS: 1336-21-6 Molecular Formula: H5NO Molecular Weight (g/mol): 35.046 InChI Key: VHUUQVKOLVNVRT-UHFFFAOYSA-N PubChem CID: 14923 ChEBI: CHEBI:18219 IUPAC Name: azanium;hydroxide SMILES: [NH4+].[OH-]
Reagecon™ Buffer Solution, pH 3.5
Manufactured in compliance with Ph. Eur. Chapter 4 (4.1.3). Reagecon™ Buffer Solution, pH 3.5 is tested at 20°C to an accuracy level of ±0.05. This calibration and control buffer is specially formulated to ensure its stability, and is ISO 17025 accredited and NIST traceable.
Buffer Solution, pH 1.00, Chem-Lab
Designed for pH measurement and maintenance in highly acidic samples. Buffer Solution, pH 1.00, Chem-Lab is a mixture of potassium chloride and hydrochloric acid with a pH level of 1.00 at 20°C. Solution is traceable to standard reference materials (SRM) from NIST.
