Molecular Probes™ Vybrant™ DyeCycle™ Green Stain

Vybrant™ DyeCycle™ Green Stain

Brand:  Molecular Probes™ V35004

Product Code. 10134622

Description

Vybrant™ DyeCycle™ Green Stain is a cell-permeable DNA dye that can be used for cell cycle analysis on a 488 nm spectral line of the argon-ion laser, which is commonly used on all flow cytometers.

Precise—accurate cell cycle analysis in living cells

Safe—low cytotoxicity for cell sorting and additional live cell experiments

Simple, robust staining protocol

View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.

Precise

Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant™ DyeCycle™ Green Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).

Low Cytotoxicity

Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant™ DyeCycle™ Green Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.

Simple, Robust Staining Protocol

For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 10⁶ cells/mL. To each tube add 2μL of Vybrant™ DyeCycle™ Green Stain. Final stain concentration is 10μM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on a flow cytometer using 488 nm excitation and green emission.

Specifications

• Concentration: 5 mM
• Detection Method: Fluorescence
• Product Type: Stain
• Emission: 488
• Format: Tube(s)
• Quantity: 200 Reactions
• Solubility: DMSO (Dimethylsulfoxide)

• Content And Storage: Contains 1 vial of Vybrant™ DyeCycle™ green stain (5 mM in DMSO). Store at ≤-20°C, desiccate and protect from light.
• For Use With (Equipment): Flow Cytometer
• Dye Type: Vybrant™ DyeCycle™ Green
• Form: Solution
• Product Line: DyeCycle™
• Shipping Condition: Room Temperature
• Sub Cellular Localization: Nucleic Acids

For Research Use Only. Not for use in diagnostic procedures.