Thermo Scientific™ Luminaris HiGreen qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Brand:  Thermo Scientific™ K0993

Product Code. 13505270

Includes: Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; Nuclease-Free Water

Description

Ready-to-use qPCR master mixes with premixed UDG optimized for SYBR Green chemistry using standard cycling protocol. Master mixes available with or without blue color.

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance
• Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity: single copy detection
• Wide linear range: accurate quantification across 9 orders of magnitude
• Reproducibility and convenience: ready-to-use 2X master mix

Specifications

• Content And Storage: Contains:
  • 10 x 1.25 mL Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 1250 x 20 μL reactions
  • 10 x 1.25 mL Nuclease-Free Water
  
  Store at -20°C.
• GC-Rich PCR Performance: High
• Polymerase: Taq DNA Polymerase
• For Use With (Equipment): BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
• Product Type: qPCR Master Mix
• For Use With (Application): Gene Expression
• Fidelity (vs. Taq): 2 X
• Sample Type: DNA, dsDNA

• Detection Method: SYBR
• PCR Method: qPCR
• Reaction Speed: Standard
• Product Line: Luminaris
• Sufficient For: 250 Reactions
• Concentration: 2X
• No. of Reactions: 1250 Reactions

For Research Use Only. Not for use in diagnostic procedures.