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Thermo Scientific™ FastDigest MnlI (IIs class)

The FastDigest MnlI restriction enzyme recognizes CCTC(7/6)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Brand:  Thermo Scientific™ FD1074

Code : 88

Additional Details : Weight : 1.51950kg

Product Code. 10561031

  • £58.75 / 50 reactions

Estimated Shipment: 07-07-2021

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Includes: Two versions of the universal FastDigest buffer are supplied with each enzyme: 10X FastDigest Buffer and 10X FastDigest Green Buffer. All FastDigest enzymes are 100% active in both buffers. Enzymes used in common downstream applications such as ligation, blunting and dephosphorylation also have 100% activity in both buffers.

Description

Description

The FastDigest MnlI restriction enzyme recognizes CCTC(7/6)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

All FastDigest enzymes are 100% active in the universal FastDigest and FastDigest Green buffers and are able to digest DNA in 5-15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. As an added convenience, the FastDigest Green Buffer allows for direct loading of the reaction mixture on a gel. FastDigest enzymes can be used to digest plasmid, genomic and viral DNA as well as PCR products and do not show star activity even in prolonged incubations.

Thermo Scientific FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

  • 100% activity of all FastDigest enzymes in the universal buffer
  • 100% buffer compatibility with downstream applications
  • Complete digestion in 5-15 minutes
  • Direct loading on gels
  • No star activity
  • 176 FastDigest enzymes available
Applications
  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP analysis
Note
The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended. For methylation sensitivity refer to product specifications.

Formulation:1 μL of enzyme (1 FDU) cleaves 1 μg of lambda DNA in 5 minutes at 37°C in 1X FastDigest Buffer.

  • Hazardous:No
  • Isoschizomers:Search for commercial isoschizomers using Research.
  • Recommended Reaction Conditions:1X FastDigest buffer or 1X FastDigest Green buffer at 37°C.
  • 1 μL of FastDigest MnlI is formulated to digest up to:
    • 1 μg of lambda DNA in 5 minutes
    • 1 μg of plasmid DNA in 5 minutes
    • 0.2 μg of PCR product in 5 minutes
    • 1 μg of genomic DNA in 5 minutes or 5 μg of genomic DNA in 30 minutes
  • Storage Condition:-20°C

Fast clone analysis; Fast preparation of DNA for cloning; Digestion of PCR products; Fast RFLP genotyping; Digestion of difficult-to-cleave DNA

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

Specifications

Specifications

FastDigest
MnlI
Yes
Yes
Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive
10x FastDigest Buffer/FastDigest Green Buffer
37° C
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