Invitrogen™ TACE/ADAM17 Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15494883	Invitrogen™ EHADAM17	96 Tests
17874170	Invitrogen™ EHADAM17X10	10 x 96 Tests
17145339	Invitrogen™ EHADAM17X5	5 x 96 Tests

Description

The Human TACE/ADAM17 ELISA quantitates Hu TACE in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TACE. Principle of the method The Human TACE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT).

Specifications

• Accession Number: P78536
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: ADAM18,CD156B,CSVP,NISBD,NISBD1,TACE
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 70 pg/mL
• Conjugate: Biotin
• Protein Family: Proteases, Peptidases & Inhibitors
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 6868
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Apoptosis
• Synonym: ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE
• Test Time: 1 hr. 20 min.