IBA Lifesciences StrepMAB-Immo Conjugate

StrepMAB-Immo conjugates are monoclonal antibodies with different fluorescent conjugates to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins

Product Code	Brand	Label or Dye
16421475	IBA Lifesciences 2-1564-050	DY-488
16451475	IBA Lifesciences 2-1567-050	DY-549
16481475	IBA Lifesciences 2-1570-050	DY-649

Description

StrepMAB-Immo fluorescenct conjugates are murine, high-affinity monoclonal antibodies, which are especially suited for detection of Strep-tag™II and Twin-Strep-tag™ fusion proteins in applications like ELISA, FACS, immunohistochemistry, and immunocytochemistry.

The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag™II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein).

Proteins containing Strep-tag™II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far, the Twin-Strep-tag™ always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK). StrepMAB-Immo is available as conjugate with different fluorescent dyes (DY-488, DY-549, DY-649).

The absorption/emission maxima of the fluorescent dyes are:
• DY-488: 493/518 nm
• DY-549: 550-560/575 nm
• DY-649: 655/676 nm

Please note: For detection of Strep-tag™II or Twin-Strep-tag™ fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin™ is recommended.

Specifications

• Capacity (Metric): 0.10 mL
• Kit Contents: Solution in PBS; 0.01 % NaN3; 1 mg/ mL BSA
• Quantity: 50 μg
• Target: Strep-tag™II and Twin-Strep-tag™

• Concentration: 0.5 mg/mL
• Content And Storage: Undiluted: 2°C to 8°C, protect from light; Diluted: -25°C to -15°C
• For Use With (Application): ELISA, FACS, immunofluorescence
• Type: Conjugate

For Research Use Only.