IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression

Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen.

Brand:  IBA Lifesciences 5-4564-001

Product Code. 15683798

Description

pESG-IBA mammalian expression vectors

pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.

• NeoR for generation of stable cells lines.
• Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
• SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
• ColE1 + Amp: for propagation in E. coli
• NeoR: neomycin resistance gene to generate stable cell lines
• BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

• Format: Liquid
• Description: pESG-IBA 164
• Includes: Neomycin resistance gene
• Promoter: Human CMV Immediate-early
• Storage Buffer: 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
• Protein Tag: Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal
• Quantity: 5 μg
• For Use With (Application): Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins

• For Use With (Equipment): StarGate™ System, Mammalian Host Cells
• Inducing Agent: SV40 large T antigen (e.g. COS1 or COS7)
• pH: 8
• Product Type: Acceptor Vector for mammalian expression
• Content And Storage: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Concentration: 250 ng/μL
• Color: Clear