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Invitrogen™ SiteClick™ Antibody Labeling Kits

Generate brightly labeled antibodies for flow cytometry, fluorescence imaging, or western blot detection using SiteClick™ Qdot™ R-PE, and Biotin Antibody Labeling Kits.

£459.00 - £560.00

Specifications

Product Type Labeling Kit
Content And Storage Store the entire kit at 2 to 6°C upon receipt
Labeling Method SiteClick Labeling System
Shipping Condition Wet Ice
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Products 10
Product Code Brand Excitation/Emission Label or Dye Labeling Scale Price Quantity & Availability  
Product Code Brand Excitation/Emission Label or Dye Labeling Scale Price Quantity & Availability  
15138735
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Invitrogen™
S10453
405/655 Qdot 655 100 μg
£540.00
Each
Estimated Shipment: 07-01-2025
to see stock.
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15108745
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Invitrogen™
S10467
496, 546, 565/578 R-PE (R-Phycoerythrin) 100 μg
£552.00
Each
Estimated Shipment: 07-01-2025
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Edge
Add to basket
 
15128735
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Invitrogen™
S10449
405/525 Qdot 525 100 μg
£532.00
Each
Estimated Shipment: 07-01-2025
to see stock.
Add to basket
 
15136585
View Documents
Invitrogen™
S10454
405/705 Qdot 705 100 μg
£534.00
1 set
Estimated Shipment: 13-01-2025
to see stock.
Add to basket
 
15148735
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Invitrogen™
S10450
405/565 Qdot 565 100 μg
£516.00
Each
Estimated Shipment: 13-01-2025
to see stock.
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15158735
Unterlagen ansehen
Invitrogen™
S10451
405/585 Qdot 585 100 μg
£522.00
Each
Estimated Shipment: 13-01-2025
to see stock.
Zum Warenkorb hinzufügen
 
15168735
Unterlagen ansehen
Invitrogen™
S10452
405/625 Qdot 625 100 μg
£560.00
Each
Estimated Shipment: 06-01-2025
to see stock.
Zum Warenkorb hinzufügen
 
15188735
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Invitrogen™
S10455
Qdot 800 100 μg
£536.00
Each
Estimated Shipment: 13-01-2025
to see stock.
Zum Warenkorb hinzufügen
 
15198735
Unterlagen ansehen
Invitrogen™
S10469
405/605 Qdot 605 100 μg
£459.00
Each
Estimated Shipment: 03-01-2025
to see stock.
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15782966
Unterlagen ansehen
Invitrogen™
S20033
Biotin 100 to 250 μg
£520.00
1 set
Estimated Shipment: 08-01-2025
to see stock.
Zum Warenkorb hinzufügen
 
Beschreibung

Beschreibung

Generate perfectly labeled antibodies with the SiteClick R-PE, Qdot, or Biotin Antibody Labeling Kits. Site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies, regardless of isotype and host species. Depending upon the label, the resulting SiteClick R-PE, Qdot, or Biotin conjugated antibody can be used in flow cytometry, fluorescence imaging, or Western blot detection. Each kit contains everything needed to label 100 μg of IgG antibody with an easy-to-follow, step-by-step protocol. Achieve highly efficient, site-specific, and reproducible labeling with the SiteClick R-PE, Qdot, or Biotin Antibody Labeling Kits.

Conventional amine or thiol labeling chemistry can result in labeling of the antigen binding domain or compromise the structure of an antibody. SiteClick labeling technology, however, attaches the label under mild conditions to the heavy chains of an IgG antibody, ensuring that the antigen binding domains remain available for binding to your antigen target. Because SiteClick Labeling Kits allow simple and gentle site-selective attachment of detection molecules to heavy chain N-linked glycans — far from the antigen-binding domain — excellent reproducibility is achieved during sequential labelings, as well from antibody to antibody. No harsh reduction steps are required, and antibody labeling is consistent and reproducible each time it is performed.

The SiteClick R-PE, Qdot, or Biotin Antibody labeling workflow consists of three steps:

1. antibody carbohydrate domain modification,
2. azide attachment to the antibody and
3. conjugation with the DIBO-modified label.

This workflow employs copper-free click chemistry to covalently link the label containing the DIBO moiety with the azide-modified antibody. Labeling antibodies with the SiteClick antibody labeling system gives you confidence that the antibody will be labeled the same way every time, with no need for reaction optimization. This means the same number of dyes are attached to each molecule, the antigen binding site is preserved, and the same results are achieved every time.

Important features of the SiteClick Qdot Antibody Labeling Kits:

• Include everything required to label 100 μg of IgG antibody (100–250 μg of IgG antibody with the SiteClick Biotin Antibody Labeling Kit)
• Easy to follow step-by-step protocol that require only a few hours hands-on time to complete.
• Highly efficient, site-specific, reproducible labeling chemistry results in high quality antibody conjugates.

Spezifikation

Spezifikation

Labeling Kit
Store the entire kit at 2 to 6°C upon receipt
SiteClick Labeling System
Wet Ice
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Produktzertifizierungen
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