Macherey-Nagel Bioanalysis™ Protino™ Ni-NTA Agarose

The method of choice for His-tag protein purification.

Product Code	Brand	Quantity
11912422	Macherey-Nagel Bioanalysis™ 745400.25	25 mL
11952192	Macherey-Nagel Bioanalysis™ 745400.500	500 mL

Description

• Enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
• Proteins from any expression system can be purified under native or denaturing conditions.
• Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions.
• Consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6% cross-linked agarose beads that are suitable for batch binding, gravity flow, and FPLC™ columns.
• The resin is precharged with Ni2+ ions and therefore ready to use.
• Technology: Immobilized metal ion affinity chromatography
• Batch binding, gravity flow column chromatography, MPLC / FPLC applications
• Matrix: 6 % beaded agarose (cross-linked), precharged with Ni2+
• Bead size: 45 to 165 μm

Specifications

• Binding Capacity: < 50 mg/mL settled agarose
• Content And Storage: 4°C to 8°C
• Target: Protein
• Ligand Type: NTA (nitrilotriacetic acid)

• Description: Aqueous suspension
• For Use With (Application): Purification of His-tag proteins
• Type: Agarose

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3
• Skin sensitiser Category 1

Hazard Statement

• H226-Flammable liquid and vapour.
• H317-May cause an allergic skin reaction.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.

Supplemental information

• MIXTURE LIST-Contains : ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU), resin suspension with Ni in