PLZF Monoclonal Antibody (9E12), PE-Cyanine7, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-9322-80

Product Code. 16330804

Description

This 9E12 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans. In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells. Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells. In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells. PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans. PLZF is expressed by innate lymphoid cells and its progenitor cells. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, growth suppression, and maintece of homeostatis. Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor. This 9E12 antibody stains mouse thymocytes brighter than Mags.21F7. This 9E12 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This can be used at less than or equal to 0.

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

Specifications

• Antigen: PLZF
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: Q05516
• Gene Symbols: ZBTB16
• Immunogen: PLFZ peptide
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry
• Clone: 9E12
• Conjugate: PE-Cyanine7
• Gene: ZBTB16
• Gene Alias: AI467657; Green's luxoid; lu; luxoid; Lx; PLZF; Polydactyly-luxate syndrome; promyelocytic leukaemia zinc finger; promyelocytic leukemia zinc finger; Promyelocytic leukemia zinc finger protein; ZBTB16; Zfp145; zinc finger and BTB domain containing 16; zinc finger and BTB domain containing 16 protein; zinc finger and BTB domain-containing protein 16; Zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); zinc finger protein PLZF; ZNF145
• Host Species: Armenian Hamster
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 235320, 7704
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

For Research Use Only.