Macherey-Nagel™ NucleoSpin™ Plasmid DNA Elution Buffer AE

Used to extract pure plasmids using column chromatography. Macherey-Nagel™ NucleoSpin™ Plasmid DNA Elution Buffer AE is designed for use with NuceloSpin™ Plasmid purification column kits. The Tris/HCl buffer is slightly alkaline and provides low iconic strength recovery conditions.

Brand:  Macherey-Nagel™ 740917.1

Product Code. 12716563

Description

NucleoSpin™ Plasmid kits feature a protocol that concludes with an elution step. Plasmid DNA Elution Buffer AE is a 5mM Tris/HCl solution with a pH of 8.5 for extracting pure plasmid DNA from the matrix of the NucleoSpin™ column under low ionic strength conditions.

Supplemental Elution Buffer AE is necessary when adapting the buffer volume to achieve a higher yield, which may be required for some downstream applications. NucleoSpin™ Plasmid kits include enough Elution Buffer AE to accommodate the standard protocol (typical recovery: 70–90%).

• Higher yield in general, esp. for larger constructs: Heat buffer, use up to 2× the buffer, incubate longer
• High yield: Perform two elution steps, which uses 2× the elution buffer, for a recovery of 90–100%
• High concentration: Use 60% volume of elution buffer for ∼130% concentration (max. yield: 80%)
• High yield and high concentration: Perform a longer incubation using 50% volume of elution buffer, then apply a standard elution step (yield: 85–100%, at a high concentration)

Specifications

• Concentration: 5mM
• pH: 8.5
• CAS: 77-86-1
• Buffer: Elution Buffer AE

• For Use With (Equipment): NucleoSpin™ Plasmid DNA kits
• Quantity: 1000 mL
• For Use With (Application): For Plasmid DNA Purification
• Product Type: Tris–HCl solution