Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 7.0

Product Code	Brand	Quantity
15464609	Thermo Scientific Chemicals J62195.AK	250 mL
15474609	Thermo Scientific Chemicals J62195.AP	500 mL

Description

PIPES (Piperazine-N,N?-bis(2-ethanesulfonic acid), is frequently used as a buffering agent in biochemistry. PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues and has a negligible capacity to bind divalent ions. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer is done.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
PIPES (Piperazine-N,N′-bis(2-ethanesulfonic acid), is frequently used as a buffering agent in biochemistry. PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues and has a negligible capacity to bind divalent ions. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer is done.

Solubility
Soluble in 1M NaOH (0.5 M at 20°C), water (∼1 g/L at 100°C), and 0.5 N NaOH (0.1 M).

Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away from strong oxidizing agents.

Chemical Identifiers

• CAS: 5625-37-6
• Molecular Weight (g/mol): 302.36
• InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N
• ChEBI: CHEBI:44933
• SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

• Molecular Formula: C8H18N2O6S2
• MDL Number: MFCD00006159
• PubChem CID: 79723
• IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid

Specifications

• CAS: 5625-37-6
• Concentration: 1.0 M
• Physical Form: Liquid
• MDL Number: MFCD00006159
• InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N
• IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
• PubChem CID: 79723
• Chemical Name or Material: PIPES buffer soln.

• Color: Colorless
• pH: 7.0
• Molecular Formula: C8H18N2O6S2
• Solubility Information: Soluble in 1M NaOH (0.5 M at 20°C),water (∽1g/L at 100°C),and 0.5 N NaOH (0.1 M).
• SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
• Molecular Weight (g/mol): 302.36
• ChEBI: CHEBI:44933

Safety and Handling

missing translation for 'tsca' : Yes

Recommended Storage : Keep cold

RUO – Research Use Only