Thermo Scientific™ Pierce™ LDS Sample Buffer, Non-Reducing (4X)

Nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.

Brand:  Thermo Scientific™ 84788

Product Code. 10725566

Description

Thermo Scientific™ Pierce LDS Sample Loading Buffer (4X) is a nonreducing lithium dodecyl sulfate sample loading buffer optimized for use with Pierce Protein SDS-PAGE Gels.

LDS Sample Buffer, Non-Reducing (4X) is a convenient sample buffer for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The buffer contains coomassie dye, enabling visualization of the electrophoretic progress by the location of the dye front. The LDS Sample Buffer, Non-Reducing (4X) may be used in denaturing gels and is compatible with both Coomassie and silver staining and Western blotting applications.

Highlights:

• Concentrated – 4X formulation provides more versatility than traditional 2X buffers

• Nonreducing – ready to use for non-reducing SDS-PAGE, or the preferred type and amount of reducing agent (e.g., DTT) can be added to produce reducing conditions

• SDS alternative – lithium dodecyl sulfate (LDS) replaces sodium dodecyl sulfate (SDS) of traditional loading buffers but functions equally well

• Physiologic pH – buffered with triethanolamine (pH 7.6) rather than Tris (pH 6.8)

• Lane marker dyes – includes trace amounts of phenol red and coomassie G250 as visible markers of the dye front

• Convenient – stable at room temperature, enabling storage at the bench where electrophoresis is performed

Specifications

• Content And Storage: Upon receipt store at room temperature.
• Concentration: 4X
• Product Type: LDS Sample Buffer
• Product Line: Pierce™

• Buffer: Sample Loading Buffers
• Gel Type: SDS-PAGE
• Gel Compatibility: SDS-PAGE Gels
• Quantity: 5 mL

Safety and Handling

Product Identifier

• LDS SAMPLE BUFFER NON-REDUCING

Signal Word

• Warning

Hazard Category

• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.

Supplemental information

• MIXTURE LIST-Contains : Triethanolamine, Sulfuric acid, monododecyl ester, lithium salt, Hydrogen chloride

For Research Use Only. Not for use in diagnostic procedures.