Thermo Scientific™ Pierce™ DAB Substrate Kit

Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.

Brand:  Thermo Scientific™ 34002

Product Code. 10006913

Description

Thermo Scientific™ Pierce DAB and the DAB Substrate Kit enable chromogenic detection of horseradish peroxidase (HRP) activity based the action of 3,3'-diaminobenzidine (DAB) in blotting and tissue staining methods.

DAB (3,3'-diaminobenzidine tetrahydrochloride; MW = 214.1) reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples. The brown precipitate is insoluble in alcohol and other organic solvents, making it an excellent substrate for immunohistochemical staining that requires the use of traditional counterstains and mounting media. The Pierce DAB Substrate Kit includes a 10X solution of DAB and Stable Peroxide Substrate Buffer. Crystalline DAB (powder) is also available.

Highlights:

• HRP substrate – for detection of horseradish peroxidase activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples

• Chromogenic – no special equipment needed for visualization; the DAB reaction produces brown bands or spots at sites of reaction with HRP-conjugated antibodies or probes

• Package options – choose powder for custom formulation with buffer and peroxide or a complete kit of preformulated solutions for immediate use

Specifications

• Description: Pierce DAB Substrate Kit
• Detection Method: Colorimetric
• Membrane Compatibility: Nitrocellulose, PVDF
• Quantity: 275 mL
• Substrate: Horseradish Peroxidase
• Sufficient For: 25 Uses at 10 mL Each

• Product Type: Substrate
• Form: Liquid
• Product Line: Pierce™
• Conjugate: HRP
• Reactivity: Horseradish Peroxidase
• Content And Storage: Sufficient For: 25 uses at 10 mL each
  • DAB (10X), 25 mL
  • Stable Peroxide Buffer, 250 mL
  
  Store kit at 4°C.

Safety and Handling

Product Identifier

• Pierce DAB Substrate Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Flammable liquid Category 2
• Specific target organ toxicity after single exposure Category 1

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H301-Toxic if swallowed.
• H311-Toxic in contact with skin.
• H331-Toxic if inhaled.
• H370-Causes damage to organs.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P353-Rinse skin with water/shower.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : methanol

For Research Use Only. Not for use in diagnostic procedures.