Thermo Scientific™ Phusion High-Fidelity DNA Polymerase Promotion

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Product Code	Brand	Content And Storage	Quantity
15727506	Thermo Scientific™ F530L	Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration. Recommended storage temperature -20°C. Stable for one year from the assay date.	500U (2U/μL)
15737506	Thermo Scientific™ F530S	Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration. Recommended storage temperature -20°C. Stable for one year from the assay date.	100U (2U/μL)
15757506	Thermo Scientific™ F531S	With High-Fidelity buffer. Recommended storage temperature -20°C. Stable for one year from the packaging date. After thawing the mix can be stored at +4°C for three months.	100 x 50uL Rxns
15747506	Thermo Scientific™ F531L	With High-Fidelity buffer. Recommended storage temperature -20°C. Stable for one year from the packaging date. After thawing the mix can be stored at +4°C for three months.	500 x 50uL Rxns
15777506	Thermo Scientific™ F532S	With GC Buffer. Recommended storage temperature -20°C. Stable for one year from the packaging date. After thawing the mix can be stored at +4°C for three months.	100 x 50uL Rxns
15767506	Thermo Scientific™ F532L	With GC Buffer. Recommended storage temperature -20°C. Stable for one year from the packaging date. After thawing the mix can be stored at +4°C for three months.	500 x 50uL Rxns
15797506	Thermo Scientific™ F534S
15787506	Thermo Scientific™ F534L

Description

Phusion DNA Polymerases set a gold standard for PCR performance. They offer a combination of characteristics that no other enzyme can match. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion DNA Polymerase is the most accurate thermostable polymerase available. This feature makes Phusion DNA Polymerases superior choice for cloning and other applications requiring high fidelity. The unique structure of the enzyme enables short protocol times, abundant yields and robust performance, even in the presence of PCR inhibitors. For the researcher, that translates to ease-of-use and convenience. In addition, Phusion DNA Polymerases also produce higher yields with lower enzyme amounts than other DNA polymerases.

New Phusion Green format is a combination of Phusion DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with excelent performance of Phusion DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Phusion High-Fidelity PCR Master Mixes are convenient 2X mixes designed to minimize the number of pipetting steps. Only template and primers need to be added by the user. Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control template and primers.

Highlights

• Extreme fidelity (error rate 4.4 x 10^-7 in Phusion HF Buffer)
• Polymerase speed allows short extension times (15 to 30 s/kb)
• Robust performance, minimal optimization needed
• Increased product yields with minimal enzyme amounts
• Direct loading on gel with Green Buffer

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.