Thermo Scientific™ pH Gradient Buffers

Product Code	Brand	Description	pH	Quantity
15125624	Thermo Scientific™ 083273	CX-1 pH Gradient Buffer A	5.6	125 mL
15135624	Thermo Scientific™ 083275	CX-1 pH Gradient Buffer B	10.2	125 mL
15165624	Thermo Scientific™ 083274	CX-1 pH Gradient Buffer Kit; 125 mL Buffer A + 125 mL Buffer B	5.6 to 10.2	125 mL + 125 mL
15145624	Thermo Scientific™ 085346	CX-1 pH Gradient Buffer A	5.6	250 mL
15155624	Thermo Scientific™ 085348	CX-1 pH Gradient Buffer B	10.2	250 mL
15121206	Thermo Scientific™ 085349	CX-1 pH Gradient Buffer Kit; 250 mL Buffer A + 250 mL Buffer B	5.6 to 10.2	250 mL + 250 mL
15870452	Thermo Scientific™ 302779	CX-1 pH Gradient Buffer A	5.6	500 mL
15880452	Thermo Scientific™ 302780	CX-1 pH Gradient Buffer B	10.2	500 mL
15195624	Thermo Scientific™ 083381	CX-1 pH Gradient Starter Kit; 125 mL Buffer A + 125 mL Buffer B MAbPac SCX-10 Column (10 μm, 4 x 250 mm)	5.6 to 10.2	125 mL + 125 mL
15185624	Thermo Scientific™ 083378	CX-1 pH Gradient High Throughput Kit; 125 mL Buffer A + 125 mL Buffer B MAbPac SCX-10 Column (5 μm, 4 x 50 mm)	5.6 to 10.2	125 mL + 125 mL
15105634	Thermo Scientific™ 083272	CX-1 pH Gradient High Resolution Kit; 125 mL Buffer A + 125 mL Buffer B MAbPac SCX-10 Column (5 μm, 4 x 250 mm)	5.6 to 10.2	125 mL + 125 mL

Description

Obtain fast and highly robust, reproducible HPLC gradients using Thermo Scientific™ CX-1 pH Gradient Buffers. Simple to optimize and easily automated, these buffers are applicable for HPLC separation of the majority of monoclonal antibody (mAb) biotherapeutics drugs. CX-1 pH Gradient Buffers are ready to use with existing HPLC columns without the need to formulate mobile phases. The CX-1 buffers save time in method development, facilitate method transfer to QA/QC for a wide range of mAb charge variants through a generic HPLC-based approach to mAb characterization, and do not require time-consuming mobile phase adjustments.

pH buffer solutions can be used to generate highly reproducible, linear pH gradients in cation exchange chromatography.

• Robust, reproducible gradients
• Predict biomolecule pH
• Simple method optimization
• Easily automated
• Applicable to the majority of MAbs

Unlike traditional cation exchange chromatography using salt gradients, it is possible to predict the pH and the expected retention of the charge variants and use a narrow pH range to get a higher resolution separation.

Specifications

• Product Line: Thermo Scientific