Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.

Brand:  Thermo Scientific™ 23030

Product Code. 11891335

Description

Thermo Scientific™ Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation.

Highlights:

• Forms covalent bonds with sulfhydryl groups at pH 6.5-7.5
• Reaction can be monitored by measuring the decrease in absorbance at 300nm

Specifications

• Chemical Reactivity: Thiol
• Label or Dye: Unreactive Group
• Product Line: Pierce™
• Spacer: Medium (10 to 30 Å)
• Quantity: 25 g
• Product Type: NEM

• Content And Storage: Upon receipt store at -20°C.
• Label Type: Blocking Groups
• Reactive Moiety: Maleimide
• Form: Solid
• Solubility: Water

Safety and Handling

Product Identifier

• N-Ethylmaleimide

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 2
• Acute toxicity Category 3
• Serious eye damage/eye irritation Category 1
• Skin corrosion/irritation Category 1 B
• Skin sensitiser Category 1

Hazard Statement

• H300-Fatal if swallowed.
• H311-Toxic in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H317-May cause an allergic skin reaction.
• H318-Causes serious eye damage.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : N-ethylmaleimide

For Research Use Only. Not for use in diagnostic procedures.