Thermo Scientific™ Kligler Iron Agar (Dehydrated)

Identify Enterobacteriaceae on the basis of double sugar fermentation and hydrogen sulphide production with this medium.

Brand:  Thermo Scientific™ CM0033B

Product Code. 10631675

Description

Identify Enterobacteriaceae on the basis of double sugar fermentation and hydrogen sulphide production using Thermo Scientific™ Oxoid™ Kligler Iron Agar (Dehydrated). Klingler Iron Agar combines the features of Kligler's Lead Acetate medium^1,2,3 and Russell's Double Sugar Agar⁴. The medium is recommended for the identification of colonies picked off from plating media such as MacConkey Agar, Bismuth Sulphite Agar, or Desoxycholate Citrate Agar, etc.

Differentiate Enterobacteriaceae on the basis of double sugar fermentation and hydrogen sulphide production with Klinger Iron Agar.

Non lactose fermenters initially produce a yellow slant and butt as a result of glucose fermentation. Once the glucose is depleted, the reactions reverts to red slants (alkaline) due to oxidation of acids. Lactose fermenting organisms produce yellow slants and butts. There is no reversion to red in the slant because enough acid is produced to maintain an acid pH under aerobic conditions. Non-fermenters produce red slants and butts. H 2 S production results in a blackening of the medium, either throughout the butt or in a ring formation near the top of the butt. Gas production is demonstrated by the presence of bubbles or cracks in the medium.

Typical reactions observed on Kligler Iron Agar after 18-24 hours at 35°C.

Organism	Slope	Butt	Gas	H2S
Shigella sonnei	Red	Yellow	-	-
Shigella dysenteriae	Red	Yellow	-	-
Salmonella typhi	Red	Yellow	-	+
Salmonella species	Red	Yellow	+	+
Enterobacter species	Red	Yellow	+	-
Klebsiella species	Yellow	Yellow	+	-
Escherichia coli	Yellow	Yellow	+	-
Proteus mirabilis	Red	Yellow	-	+
Morganella species	Red	Yellow	V	-
Citrobacter freundii	Yellow	Yellow	+	+
Yersinia species	Red	Yellow	V	-

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Specifications

• Description: Kligler Iron Agar
• Product Type: Agar
• Yield: For 9.1L medium

• Form: Powder
• Quantity: 500 g

For Laboratory Use Only