Macherey-Nagel™ NucleoSpin™ Plasmid

Prepare small scale high-purity plasmid DNA rapidly. NucleoSpin™ Plasmid comes with or without a cap lid.

Product Code	Brand	No. of Reactions	Includes
11942502	Macherey-Nagel™ 740588.10	10 Preps	Lid
11932392	Macherey-Nagel™ 740588.50	50 Preps	Lid
12353358	Macherey-Nagel™ 740588.250	250 Preps	Lid
11912252	Macherey-Nagel™ 740499.250	250	No Lid
11942362	Macherey-Nagel™ 740499.50	50	No Lid

Description

Features:

• For rapid small scale preparation high-purity plasmid DNA.
• Fast procedure – 6 Minipreps in 25 min.
• High capacity – up to 50μg of plasmid DNA.
• Chaotropic salt wash buffer – extra pure DNA.
   

Applications:

• High-copy plasmids from E. coli.
• Low-copy plasmids, P1 constructs, or cosmids (increased buffer volumes required).
• Plasmid DNA from Gram-positive bacteria and plasmid DNA clean-up from reaction mixtures.
• Supplementary protocol for M13 DNA available on request.
• Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis.

Specifications

• Format: Column
• Sequencing Type: DNA and RNA Sequencing
• Product Type: Plasmid Columns
• Sample Volume: 2 to 10 mL

• Isolation Technology: Silica Membrane Technology
• For Use With (Application): High-copy plasmids from E. coli
• Quantity: 2 mL
• Size: <50 kbp

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H336-May cause drowsiness or dizziness.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt/guanidine hydrochloride/2-pr