Macherey-Nagel™ NucleoSpin™ 96 Pasmid Kit

Designed for manual or automated large-scale purification of high-copy plasmid DNA from E.coli in the 96-well plate format. Macherey-Nagel™ NucleoSpin™ 96 Pasmid Kit is an ultra-fast and easy-to-use kit that supplies all core and accessory plates, buffers and RNase A for the highest convenience.

Product Code	Brand	No. of Reactions	Includes
12857321	Macherey-Nagel™ 740625.24	24 x 96 Preps	NucleoSpin Plasmid Filter Plate (24), NucleoSpin Plasmid Binding Plate (24), MN Wash Plate (24), Culture plate with Gas-permeable Foil (24), Elution Plate with Self-adhering Foil (24), RNase A (6x60mg), Resuspension Buffer A1 (6x150mL), Lysis Buffer A2 (6x150mL), Neutralization Buffer A3 (6x200mL), Wash Buffer AW (6x400mL), Wash Buffer A4 Concentrate (6x200mL), Elution Buffer AE (6x125mL)
12830741	Macherey-Nagel™ 740625.4	4 x 96 preps	NucleoSpin Plasmid Filter Plate (4), NucleoSpin Plasmid Binding Plate (4), MN Wash Plate (4), Culture plate with Gas-permeable Foil (4), Elution Plate with Self-adhering Foil (4), RNase A (60mg), Resuspension Buffer A1 (150mL), Lysis Buffer A2 (150mL), Neutralization Buffer A3 (200mL), Wash Buffer AW (400mL), Wash Buffer A4 (Concentrate) (200mL), Elution Buffer AE (125mL)

Description

NucleoSpin™ 96 Pasmid kits enable the high-throughput isolation of sequencing-grade plasmid DNA from E. coli. The proven 96-well plate format allows for easy automation on common liquid handling instruments or for manual processing. Up to 96 samples can be processed simultaneously, typically within less than 45 minutes. Uses include isolation of high-copy plasmids, purification of cosmids and reliable downstream applications, such as cloning, sequencing, PCR, transformation, and restriction analysis.

• Time-saving parallel isolation of plasmid DNA
• Processing of up to 5mL bacterial culture
• 96-Well filter plate for convenient filtration of bacterial lysates
• Suitable for manual or automated processing under vacuum or by centrifugation
• Innovative MN Wash Plate minimizes risk of cross-contamination
• A₂₆₀/A₂₈₀: 1.70–1.85; Binding capacity: 20μg

Specifications

• Format: 96-well Plate
• Elution Volume: Elution volume: 75–150μL
• For Use With (Equipment): Liquid handling instruments
• Product Type: NucleoSpin™ 96 Plasmid Kit
• Sample Volume: 1–5mL
• Yield: 4–6μg plasmid per 1mL E. coli culture
  Maximum plasmid size: <15 kbp

• Sample Type: E. coli Culture
• Final Product Type: Sequencing grade plasmid DNA
• For Use With (Application): Manual and automated processing under vacuum or by centrifugation
• Quantity: 1 Kit
• Test Time: Preparation time: 45 min. / plate

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Specific target organ toxicity Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H336-May cause drowsiness or dizziness.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt / guanidine hydrochloride, 2