Macherey-Nagel™ NucleoBond™ Xtra Midi Plus EF

Purify endotoxin-free plasmid DNA with high yield. The NucleoBond™ Xtra Midi Plus EF Kit represents the new generation of anion exchanger allowing high yield, ultra-fast. The Plus kit contains NucleoBond™ Finalizers to speed up DNA precipitation.

Product Code	Brand	No. of Reactions
11932472	Macherey-Nagel™ 740422.10	10
12718422	Macherey-Nagel™ 740422.50	50

Description

Features

• Endotoxin-free
• Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells
• Patented endotoxin removal by additional washing step – no extra ice incubation necessary
• Optimized column design – Midi prep in 45 min.
• Column filter included – high filter flow-rates, parallel lysate clearing and loading onto the column
• Improved silica material – typical yields of 400μg (Midi)
• NucleoBond™ Xtra EF kits contain NucleoBond™ Finalizers to speed up DNA precipitation

Applications

• High-copy plasmids from E. coli
• Low-copy plasmids from E. coli (increased buffer volumes required)

Specifications

• Buffer: Yes
• Isolation Technology: Anion Exchange Chromatography Technology
• Size: <300kbp

• Format: Midi Gravity-flow Column
• RNase: Yes
• Yield: 400μg

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1

Hazard Statement

• H226-Flammable liquid and vapour.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : 2-propanol/ethanol (diluted < 20 %)/sodium hydroxide solution (diluted < 2 %), dodecyl sulfate, sodi