Macherey-Nagel™ NucleoBond™ Xtra Maxi Kits

Purify transfection-grade plasmid DNA with high yield. The NucleoBond™ Xtra Maxi Kit represents the new generation of anion exchanger. The optimized design results in high yield and ultra-fast processing.

Brand:  Macherey-Nagel™ 740414.10

Product Code. 12792603

Description

Features

• Optimized column design—Silica resin packed between filters, with prep in just 75 minutes
• Column filter included—High filter flow rates, parallel lysate clearing, and loading onto the column
• Improved silica material—Typical yields of 1,000μg and can be used over a wide pH range
• Proven anion-exchange technology—Transfection-grade plasmid DNA
• Kits contain: NucleoBond™ Xtra Maxi columns and filter, RNase A, buffers (RES, LYS, NEU, EQU, WASH, ELU) and plastic washers

Applications

• High-copy plasmids from E. coli
• Low-copy plasmids from E. coli (increased buffer volumes required)

Specifications

• Buffer: Yes
• Isolation Technology: Anion Exchange Chromatography Technology
• Purity: A₂₆₀/A₂₈₀:1.80–1.95
• Sample Volume: <600mL (high copy), <1200mL (low copy)
• Test Time: 75 min./prep

• Format: Maxi Gravity-flow Column
• No. of Reactions: 10
• RNase: Yes
• Size: <300kbp
• Yield: 1000μg

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1

Hazard Statement

• H226-Flammable liquid and vapour.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : 2-propanol/sodium hydroxide solution (diluted < 2 %),dodecylsulfate, sodium salt/ethanol (diluted <